Development of chromosome-based T7 RNA polymerase and orthogonal T7 promoter circuit in Escherichia coli W3110 as a cell factory

Development of chromosome-based T7 RNA polymerase and orthogonal T7 promoter circuit in Escherichia coli W3110 as a cell factory

Abstract Background Orthogonal T7 RNA polymerase (T7RNAP) and T7 promoter is a powerful genetic element to mediate protein expression in different cells. Among all, Escherichia coli possess advantages of fast growth rate, easy for culture and comprehensive elements for genetic engineering. As E. col...

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Bibliographic Details
Main Authors: Wan-Wen Ting, Shih-I Tan, I-Son Ng
Format: Article
Language:English
Published: SpringerOpen 2020-10-01
Series:Bioresources and Bioprocessing
Subjects:
E. coli W3110
T7 RNA polymerase
T7 promoter
Recombinant protein
Cadaverine
Online Access:http://link.springer.com/article/10.1186/s40643-020-00342-6

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http://link.springer.com/article/10.1186/s40643-020-00342-6

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