Site Selective and Efficient Spin Labeling of Proteins with a Maleimide-Functionalized Trityl Radical for Pulsed Dipolar EPR Spectroscopy

Site Selective and Efficient Spin Labeling of Proteins with a Maleimide-Functionalized Trityl Radical for Pulsed Dipolar EPR Spectroscopy

Pulsed dipolar electron paramagnetic resonance spectroscopy (PDS) in combination with site-directed spin labeling (SDSL) of proteins and oligonucleotides is a powerful tool in structural biology. Instead of using the commonly employed <i>gem</i>-dimethyl-nitroxide labels, triarylmethyl (...

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Bibliographic Details
Main Authors: J. Jacques Jassoy, Caspar A. Heubach, Tobias Hett, Frédéric Bernhard, Florian R. Haege, Gregor Hagelueken, Olav Schiemann
Format: Article
Language:English
Published: MDPI AG 2019-07-01
Series:Molecules
Subjects:
proteins
distance measurements
EPR
DQC
PELDOR
SIFTER
Online Access:https://www.mdpi.com/1420-3049/24/15/2735

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