Overexpression of Thioredoxin-1 Blocks Morphine-Induced Conditioned Place Preference Through Regulating the Interaction of γ-Aminobutyric Acid and Dopamine Systems

Overexpression of Thioredoxin-1 Blocks Morphine-Induced Conditioned Place Preference Through Regulating the Interaction of γ-Aminobutyric Acid and Dopamine Systems

Morphine is one kind of opioid, which is currently the most effective widely utilized pain relieving pharmaceutical. Long-term administration of morphine leads to dependence and addiction. Thioredoxin-1 (Trx-1) is an important redox regulating protein and works as a neurotrophic cofactor. Our previo...

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Main Authors: Xiang Li, Mengbing Huang, Lihua Yang, Ningning Guo, Xiaoyan Yang, Zhimin Zhang, Ming Bai, Lu Ge, Xiaoshuang Zhou, Ye Li, Jie Bai
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-05-01
Series:Frontiers in Neurology
Subjects:
thioredoxin-1
morphine
ventral tegmental area
nucleus accumbens
conditioned place preference
Online Access:http://journal.frontiersin.org/article/10.3389/fneur.2018.00309/full
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spelling doaj-0fe23305abbd429084d3465de6d2c4fa2020-11-24T23:52:56ZengFrontiers Media S.A.Frontiers in Neurology1664-22952018-05-01910.3389/fneur.2018.00309354399Overexpression of Thioredoxin-1 Blocks Morphine-Induced Conditioned Place Preference Through Regulating the Interaction of γ-Aminobutyric Acid and Dopamine SystemsXiang Li0Xiang Li1Mengbing Huang2Mengbing Huang3Lihua Yang4Lihua Yang5Ningning Guo6Ningning Guo7Xiaoyan Yang8Zhimin Zhang9Ming Bai10Lu Ge11Xiaoshuang Zhou12Ye Li13Jie Bai14Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, ChinaLaboratory of Molecular Neurobiology, Medical Faculty, Kunming University of Science and Technology, Kunming, ChinaFaculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, ChinaLaboratory of Molecular Neurobiology, Medical Faculty, Kunming University of Science and Technology, Kunming, ChinaFaculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, ChinaLaboratory of Molecular Neurobiology, Medical Faculty, Kunming University of Science and Technology, Kunming, ChinaFaculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, ChinaLaboratory of Molecular Neurobiology, Medical Faculty, Kunming University of Science and Technology, Kunming, ChinaLaboratory of Molecular Neurobiology, Medical Faculty, Kunming University of Science and Technology, Kunming, ChinaLaboratory of Molecular Neurobiology, Medical Faculty, Kunming University of Science and Technology, Kunming, ChinaLaboratory of Molecular Neurobiology, Medical Faculty, Kunming University of Science and Technology, Kunming, ChinaLaboratory of Molecular Neurobiology, Medical Faculty, Kunming University of Science and Technology, Kunming, ChinaLaboratory of Molecular Neurobiology, Medical Faculty, Kunming University of Science and Technology, Kunming, ChinaLaboratory of Molecular Neurobiology, Medical Faculty, Kunming University of Science and Technology, Kunming, ChinaLaboratory of Molecular Neurobiology, Medical Faculty, Kunming University of Science and Technology, Kunming, ChinaMorphine is one kind of opioid, which is currently the most effective widely utilized pain relieving pharmaceutical. Long-term administration of morphine leads to dependence and addiction. Thioredoxin-1 (Trx-1) is an important redox regulating protein and works as a neurotrophic cofactor. Our previous study showed that geranylgeranylaceton, an inducer of Trx-1 protected mice from rewarding effects induced by morphine. However, whether overexpression of Trx-1 can block morphine-induced conditioned place preference (CPP) in mice is still unknown. In this study, we first examined whether overexpression of Trx-1 affects the CPP after morphine training and further examined the dopamine (DA) and γ-aminobutyric acid (GABA) systems involved in rewarding effects. Our results showed that morphine-induced CPP was blocked in Trx-1 overexpression transgenic (TG) mice. Trx-1 expression was induced by morphine in the ventral tegmental area (VTA) and nucleus accumbens (NAc) in wild-type (WT) mice, which was not induced in Trx-1 TG mice. The DA level and expressions of tyrosine hydroxylase (TH) and D1 were induced by morphine in WT mice, which were not induced in Trx-1 TG mice. The GABA level and expression of GABABR were decreased by morphine, which were restored in Trx-1 TG mice. Therefore, Trx-1 may play a role in blocking CPP induced by morphine through regulating the expressions of D1, TH, and GABABR in the VTA and NAc.http://journal.frontiersin.org/article/10.3389/fneur.2018.00309/fullthioredoxin-1morphineventral tegmental areanucleus accumbensconditioned place preference
collection DOAJ
language English
format Article
sources DOAJ
author Xiang Li
Xiang Li
Mengbing Huang
Mengbing Huang
Lihua Yang
Lihua Yang
Ningning Guo
Ningning Guo
Xiaoyan Yang
Zhimin Zhang
Ming Bai
Lu Ge
Xiaoshuang Zhou
Ye Li
Jie Bai
spellingShingle Xiang Li
Xiang Li
Mengbing Huang
Mengbing Huang
Lihua Yang
Lihua Yang
Ningning Guo
Ningning Guo
Xiaoyan Yang
Zhimin Zhang
Ming Bai
Lu Ge
Xiaoshuang Zhou
Ye Li
Jie Bai
Overexpression of Thioredoxin-1 Blocks Morphine-Induced Conditioned Place Preference Through Regulating the Interaction of γ-Aminobutyric Acid and Dopamine Systems
Frontiers in Neurology
thioredoxin-1
morphine
ventral tegmental area
nucleus accumbens
conditioned place preference
author_facet Xiang Li
Xiang Li
Mengbing Huang
Mengbing Huang
Lihua Yang
Lihua Yang
Ningning Guo
Ningning Guo
Xiaoyan Yang
Zhimin Zhang
Ming Bai
Lu Ge
Xiaoshuang Zhou
Ye Li
Jie Bai
author_sort Xiang Li
title Overexpression of Thioredoxin-1 Blocks Morphine-Induced Conditioned Place Preference Through Regulating the Interaction of γ-Aminobutyric Acid and Dopamine Systems
title_short Overexpression of Thioredoxin-1 Blocks Morphine-Induced Conditioned Place Preference Through Regulating the Interaction of γ-Aminobutyric Acid and Dopamine Systems
title_full Overexpression of Thioredoxin-1 Blocks Morphine-Induced Conditioned Place Preference Through Regulating the Interaction of γ-Aminobutyric Acid and Dopamine Systems
title_fullStr Overexpression of Thioredoxin-1 Blocks Morphine-Induced Conditioned Place Preference Through Regulating the Interaction of γ-Aminobutyric Acid and Dopamine Systems
title_full_unstemmed Overexpression of Thioredoxin-1 Blocks Morphine-Induced Conditioned Place Preference Through Regulating the Interaction of γ-Aminobutyric Acid and Dopamine Systems
title_sort overexpression of thioredoxin-1 blocks morphine-induced conditioned place preference through regulating the interaction of γ-aminobutyric acid and dopamine systems
publisher Frontiers Media S.A.
series Frontiers in Neurology
issn 1664-2295
publishDate 2018-05-01
description Morphine is one kind of opioid, which is currently the most effective widely utilized pain relieving pharmaceutical. Long-term administration of morphine leads to dependence and addiction. Thioredoxin-1 (Trx-1) is an important redox regulating protein and works as a neurotrophic cofactor. Our previous study showed that geranylgeranylaceton, an inducer of Trx-1 protected mice from rewarding effects induced by morphine. However, whether overexpression of Trx-1 can block morphine-induced conditioned place preference (CPP) in mice is still unknown. In this study, we first examined whether overexpression of Trx-1 affects the CPP after morphine training and further examined the dopamine (DA) and γ-aminobutyric acid (GABA) systems involved in rewarding effects. Our results showed that morphine-induced CPP was blocked in Trx-1 overexpression transgenic (TG) mice. Trx-1 expression was induced by morphine in the ventral tegmental area (VTA) and nucleus accumbens (NAc) in wild-type (WT) mice, which was not induced in Trx-1 TG mice. The DA level and expressions of tyrosine hydroxylase (TH) and D1 were induced by morphine in WT mice, which were not induced in Trx-1 TG mice. The GABA level and expression of GABABR were decreased by morphine, which were restored in Trx-1 TG mice. Therefore, Trx-1 may play a role in blocking CPP induced by morphine through regulating the expressions of D1, TH, and GABABR in the VTA and NAc.
topic thioredoxin-1
morphine
ventral tegmental area
nucleus accumbens
conditioned place preference
url http://journal.frontiersin.org/article/10.3389/fneur.2018.00309/full
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