Avaliação in vitro da biocompatibilidade dos braquiópodos para a elaboração de um novo material

The purpose of this study was to carry out an in vitro evaluation of the citotoxity of conchs, to prepare a new material with calcium carbonate or calcium phosphate. The conchs were triturates and rained in fine drops. The material were placed in glass slides and after placed in cells cultured...

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Main Authors: José Reis dos Santos, Kristianne Santos Porta Fernandes, Manoela Domingues Martins, Sandra Kalil Bussadori, Elaine Marcílio Santos
Format: Article
Language:Portuguese
Published: Universidade Nove de Julho 2006-01-01
Series:ConScientiae Saúde
Online Access:http://www.redalyc.org/articulo.oa?id=92900512
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spelling doaj-0fa88fb6ecb048eab6cadb5098853e682020-11-24T20:58:11ZporUniversidade Nove de Julho ConScientiae Saúde1677-10281983-93242006-01-0159196Avaliação in vitro da biocompatibilidade dos braquiópodos para a elaboração de um novo materialJosé Reis dos SantosKristianne Santos Porta FernandesManoela Domingues MartinsSandra Kalil BussadoriElaine Marcílio SantosThe purpose of this study was to carry out an in vitro evaluation of the citotoxity of conchs, to prepare a new material with calcium carbonate or calcium phosphate. The conchs were triturates and rained in fine drops. The material were placed in glass slides and after placed in cells cultured. We used cell line NIH3T3 and gingival fibroblasts plaqued 1 x 104 cells in Petri dish. The counting was realized in optic microscopoic in one, three, five and seven days and zero, four, eight and 12 hours. After the contact of the material in cells cultured, the cells number were determined by counting the cells using Trypan blue dye exclusing assay. In both tests the material allowed cellular growing. The results conclude that: the material wasnt citotoxity in vitro in fibroblasts cultured.http://www.redalyc.org/articulo.oa?id=92900512
collection DOAJ
language Portuguese
format Article
sources DOAJ
author José Reis dos Santos
Kristianne Santos Porta Fernandes
Manoela Domingues Martins
Sandra Kalil Bussadori
Elaine Marcílio Santos
spellingShingle José Reis dos Santos
Kristianne Santos Porta Fernandes
Manoela Domingues Martins
Sandra Kalil Bussadori
Elaine Marcílio Santos
Avaliação in vitro da biocompatibilidade dos braquiópodos para a elaboração de um novo material
ConScientiae Saúde
author_facet José Reis dos Santos
Kristianne Santos Porta Fernandes
Manoela Domingues Martins
Sandra Kalil Bussadori
Elaine Marcílio Santos
author_sort José Reis dos Santos
title Avaliação in vitro da biocompatibilidade dos braquiópodos para a elaboração de um novo material
title_short Avaliação in vitro da biocompatibilidade dos braquiópodos para a elaboração de um novo material
title_full Avaliação in vitro da biocompatibilidade dos braquiópodos para a elaboração de um novo material
title_fullStr Avaliação in vitro da biocompatibilidade dos braquiópodos para a elaboração de um novo material
title_full_unstemmed Avaliação in vitro da biocompatibilidade dos braquiópodos para a elaboração de um novo material
title_sort avaliação in vitro da biocompatibilidade dos braquiópodos para a elaboração de um novo material
publisher Universidade Nove de Julho
series ConScientiae Saúde
issn 1677-1028
1983-9324
publishDate 2006-01-01
description The purpose of this study was to carry out an in vitro evaluation of the citotoxity of conchs, to prepare a new material with calcium carbonate or calcium phosphate. The conchs were triturates and rained in fine drops. The material were placed in glass slides and after placed in cells cultured. We used cell line NIH3T3 and gingival fibroblasts plaqued 1 x 104 cells in Petri dish. The counting was realized in optic microscopoic in one, three, five and seven days and zero, four, eight and 12 hours. After the contact of the material in cells cultured, the cells number were determined by counting the cells using Trypan blue dye exclusing assay. In both tests the material allowed cellular growing. The results conclude that: the material wasnt citotoxity in vitro in fibroblasts cultured.
url http://www.redalyc.org/articulo.oa?id=92900512
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