Avaliação in vitro da biocompatibilidade dos braquiópodos para a elaboração de um novo material
The purpose of this study was to carry out an in vitro evaluation of the citotoxity of conchs, to prepare a new material with calcium carbonate or calcium phosphate. The conchs were triturates and rained in fine drops. The material were placed in glass slides and after placed in cells cultured...
Main Authors: | , , , , |
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Format: | Article |
Language: | Portuguese |
Published: |
Universidade Nove de Julho
2006-01-01
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Series: | ConScientiae Saúde |
Online Access: | http://www.redalyc.org/articulo.oa?id=92900512 |
Summary: | The purpose of this study was to carry out an
in vitro evaluation of the citotoxity of conchs,
to prepare a new material with calcium carbonate
or calcium phosphate. The conchs were
triturates and rained in fine drops. The material
were placed in glass slides and after placed
in cells cultured. We used cell line NIH3T3
and gingival fibroblasts plaqued 1 x 104 cells
in Petri dish. The counting was realized in optic
microscopoic in one, three, five and seven
days and zero, four, eight and 12 hours. After
the contact of the material in cells cultured,
the cells number were determined by counting
the cells using Trypan blue dye exclusing
assay. In both tests the material allowed cellular
growing. The results conclude that: the
material wasnt citotoxity in vitro in fibroblasts
cultured. |
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ISSN: | 1677-1028 1983-9324 |