A multiplex qPCR TaqMan-assay to detect fungal antagonism between Trichoderma atroviride (Hypocreaceae) and Botrytis cinerea (Sclerotiniaceae) in blackberry fruits using a de novo tef1-α- and an IGS-sequence based probes

A multiplex qPCR TaqMan-assay to detect fungal antagonism between Trichoderma atroviride (Hypocreaceae) and Botrytis cinerea (Sclerotiniaceae) in blackberry fruits using a de novo tef1-α- and an IGS-sequence based probes

The aim of this study was to design a Trichoderma atroviride-specific qPCR oligo set, evaluate its specificity, and standardize a methodology that quantifies antagonism against Botrytis cinerea in blackberry fruits (Rubus adenotrichos Schltdl.). Primers and probe were designed based on the nuclear t...

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Bibliographic Details
Main Authors: Irena Hilje-Rodríguez, Federico J. Albertazzi, German Rivera-Coto, Ramón Molina-Bravo
Format: Article
Language:English
Published: Elsevier 2020-09-01
Series:Biotechnology Reports
Subjects:
Biological control
Molecular quantification
Hydrolysis probe
qPCR
Rubus adenotrichos
Online Access:http://www.sciencedirect.com/science/article/pii/S2215017X19305454

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http://www.sciencedirect.com/science/article/pii/S2215017X19305454

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