Antagonistic activity of antibiotic producing Streptomyces sp. against fish and human pathogenic bacteria

• Main Authors: Nazmul Hossain, Mahbubur Rahman
• Format: Article
• Language: English
• Published: Instituto de Tecnologia do Paraná (Tecpar) 2014-04-01
• Series: Brazilian Archives of Biology and Technology
• Subjects: Streptomyces sp.; antagonistic activity; cross streak method; antibiotic
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200011&lng=en&tlng=en

In this study, attempts were made to isolate Streptomyces sp. from soil samples of two different regions of Bangladesh and evaluate their antagonistic activity against fish and human pathogenic bacteria. A total of 10 isolates were identified as Streptomyces sp. based on several morphological, physiological and biochemical tests. Cross streak method was used to observe the antagonistic activity of the Streptomyces sp. isolates against different fish pathogens belonging to the genus Aeromonas, Pseudomonas and Edwardsiella and human clinical isolates belonging to the genus Klebsiella, Salmonella and Streptococcus. Seven Streptomyces sp. isolates showed antagonism against both fish and human pathogenic bacteria. Four isolates viz., N24, N26, N28 and N47 showed broad spectrum of antagonistic activity (80-100%) against all genera of fish and human pathogenic bacteria. The isolate N49 exhibited highest spectrum of antagonism against all fish pathogens (90-100%) but comparatively lower degree of antagonism against human pathogens (50-60%). Rest of the two isolates (N21 and N23) showed variability in their antagonism. Results showed that broad spectrum antibiotic(s) could be developed from the isolates N24, N26, N28 and N47against several human and fish pathogens. The isolate N49 could be a potential source of antibiotic, especially for fish pathogenic bacteria.