Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell Killing

Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell Killing

CAR-NK cells may represent a valuable tool, complementary to CAR-T cells, in adoptive immunotherapy of leukemia and solid tumors. However, gene transfer to human NK cells is a challenging task, particularly with non-virus-based techniques. Here, we describe a new procedure allowing efficient electro...

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Main Authors: Tiziano Ingegnere, Francesca Romana Mariotti, Andrea Pelosi, Concetta Quintarelli, Biagio De Angelis, Nicola Tumino, Francesca Besi, Claudia Cantoni, Franco Locatelli, Paola Vacca, Lorenzo Moretta
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-04-01
Series:Frontiers in Immunology
Subjects:
Chimeric Antigen Receptors
chemokine receptors
NK cells
adoptive immunotherapy
electroporation
Online Access:https://www.frontiersin.org/article/10.3389/fimmu.2019.00957/full
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spelling doaj-0f7fca48d97c4ed88fde1ef364fdad1f2020-11-25T01:08:20ZengFrontiers Media S.A.Frontiers in Immunology1664-32242019-04-011010.3389/fimmu.2019.00957440709Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell KillingTiziano Ingegnere0Francesca Romana Mariotti1Andrea Pelosi2Concetta Quintarelli3Concetta Quintarelli4Biagio De Angelis5Nicola Tumino6Francesca Besi7Claudia Cantoni8Franco Locatelli9Paola Vacca10Lorenzo Moretta11Immunology Research Area, IRCSS Bambino Gesù Pediatric Hospital, Rome, ItalyImmunology Research Area, IRCSS Bambino Gesù Pediatric Hospital, Rome, ItalyImmunology Research Area, IRCSS Bambino Gesù Pediatric Hospital, Rome, ItalyDepartment of Hematology/Oncology, IRCCS Ospedale Pediatrico Bambino Gesù, Rome, ItalyDepartment of “Medicina Clinica e Chirurgia”, University of Naples Federico II, Naples, ItalyDepartment of Hematology/Oncology, IRCCS Ospedale Pediatrico Bambino Gesù, Rome, ItalyImmunology Research Area, IRCSS Bambino Gesù Pediatric Hospital, Rome, ItalyImmunology Research Area, IRCSS Bambino Gesù Pediatric Hospital, Rome, ItalyDepartment of Experimental Medicine and Center of Excellence for Biomedical Research, University of Genoa and Istituto G. Gaslini, Genoa, ItalyDepartment of Hematology/Oncology, IRCCS Ospedale Pediatrico Bambino Gesù, Rome, ItalyImmunology Research Area, IRCSS Bambino Gesù Pediatric Hospital, Rome, ItalyImmunology Research Area, IRCSS Bambino Gesù Pediatric Hospital, Rome, ItalyCAR-NK cells may represent a valuable tool, complementary to CAR-T cells, in adoptive immunotherapy of leukemia and solid tumors. However, gene transfer to human NK cells is a challenging task, particularly with non-virus-based techniques. Here, we describe a new procedure allowing efficient electroporation-based transfection of plasmid DNA, including CAR and CCR7 genes, in resting or cytokine-expanded human NK cell populations and NK-92 cell line. This procedure may offer a suitable platform for a safe and effective use of CAR-NK cells in adoptive immunotherapy of cancer.https://www.frontiersin.org/article/10.3389/fimmu.2019.00957/fullChimeric Antigen Receptorschemokine receptorsNK cellsadoptive immunotherapyelectroporation
collection DOAJ
language English
format Article
sources DOAJ
author Tiziano Ingegnere
Francesca Romana Mariotti
Andrea Pelosi
Concetta Quintarelli
Concetta Quintarelli
Biagio De Angelis
Nicola Tumino
Francesca Besi
Claudia Cantoni
Franco Locatelli
Paola Vacca
Lorenzo Moretta
spellingShingle Tiziano Ingegnere
Francesca Romana Mariotti
Andrea Pelosi
Concetta Quintarelli
Concetta Quintarelli
Biagio De Angelis
Nicola Tumino
Francesca Besi
Claudia Cantoni
Franco Locatelli
Paola Vacca
Lorenzo Moretta
Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell Killing
Frontiers in Immunology
Chimeric Antigen Receptors
chemokine receptors
NK cells
adoptive immunotherapy
electroporation
author_facet Tiziano Ingegnere
Francesca Romana Mariotti
Andrea Pelosi
Concetta Quintarelli
Concetta Quintarelli
Biagio De Angelis
Nicola Tumino
Francesca Besi
Claudia Cantoni
Franco Locatelli
Paola Vacca
Lorenzo Moretta
author_sort Tiziano Ingegnere
title Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell Killing
title_short Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell Killing
title_full Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell Killing
title_fullStr Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell Killing
title_full_unstemmed Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell Killing
title_sort human car nk cells: a new non-viral method allowing high efficient transfection and strong tumor cell killing
publisher Frontiers Media S.A.
series Frontiers in Immunology
issn 1664-3224
publishDate 2019-04-01
description CAR-NK cells may represent a valuable tool, complementary to CAR-T cells, in adoptive immunotherapy of leukemia and solid tumors. However, gene transfer to human NK cells is a challenging task, particularly with non-virus-based techniques. Here, we describe a new procedure allowing efficient electroporation-based transfection of plasmid DNA, including CAR and CCR7 genes, in resting or cytokine-expanded human NK cell populations and NK-92 cell line. This procedure may offer a suitable platform for a safe and effective use of CAR-NK cells in adoptive immunotherapy of cancer.
topic Chimeric Antigen Receptors
chemokine receptors
NK cells
adoptive immunotherapy
electroporation
url https://www.frontiersin.org/article/10.3389/fimmu.2019.00957/full
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