Pyramiding disease resistance genes in elite winter wheat germplasm for Western Canada

We report on pyramiding different disease resistance genes against fungal pathogens in Canadian winter wheat germplasm based on available DNA markers and gene sequences. Genetic resistance represents a safe, economical and ecological method for protecting plants, growers and the health of consumers....

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Main Authors: André Laroche, Michele Frick, Robert J. Graf, Jamie Larsen, John D. Laurie
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2019-12-01
Series:Crop Journal
Online Access:http://www.sciencedirect.com/science/article/pii/S2214514119301230
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spelling doaj-0f7aa45261304900bd251305285ed5982021-04-02T14:49:35ZengKeAi Communications Co., Ltd.Crop Journal2214-51412019-12-0176739749Pyramiding disease resistance genes in elite winter wheat germplasm for Western CanadaAndré Laroche0Michele Frick1Robert J. Graf2Jamie Larsen3John D. Laurie4Agriculture and Agri-Food Canada, Lethbridge Research and Development Centre, Lethbridge, Alberta T1J 4B1, Canada; Corresponding author.Agriculture and Agri-Food Canada, Lethbridge Research and Development Centre, Lethbridge, Alberta T1J 4B1, CanadaAgriculture and Agri-Food Canada, Lethbridge Research and Development Centre, Lethbridge, Alberta T1J 4B1, CanadaAgriculture and Agri-Food Canada, Lethbridge Research and Development Centre, Lethbridge, Alberta T1J 4B1, Canada; Agriculture and Agri-Food Canada, Harrow Research and Development Centre, 2585 County Road 20, Harrow, Ontario N0R 1G0, CanadaAgriculture and Agri-Food Canada, Lethbridge Research and Development Centre, Lethbridge, Alberta T1J 4B1, CanadaWe report on pyramiding different disease resistance genes against fungal pathogens in Canadian winter wheat germplasm based on available DNA markers and gene sequences. Genetic resistance represents a safe, economical and ecological method for protecting plants, growers and the health of consumers. Major diseases of wheat on the Canadian Prairies are common bunt, rusts (leaf, stem and stripe) and Fusarium head blight. Over the years markers for resistance genes against these diseases have been identified and used by the international wheat community. We describe markers that we have used to pyramid different resistance genes and indicate their presence in Canadian winter wheat cultivars issued from the winter wheat breeding program at the Agriculture and Agri-Food Canada, Lethbridge Research and Development Centre, the only winter wheat breeding program in Western Canada actively delivering new varieties for all regions of the Canadian Prairies. The sources of resistance and identities of PCR primers and amplification conditions are indicated to enable the transfer and pyramiding of different resistance (R) genes to breeding lines. We conclude by reviewing new tools for identifying R genes in wheat and indicate how mutagenesis and gene editing can help future efforts to extend the protection offered by known R genes. Keywords: Bunt, Gene pyramiding, Wheat rusts, Marker-assisted selection, Triticum aestivumhttp://www.sciencedirect.com/science/article/pii/S2214514119301230
collection DOAJ
language English
format Article
sources DOAJ
author André Laroche
Michele Frick
Robert J. Graf
Jamie Larsen
John D. Laurie
spellingShingle André Laroche
Michele Frick
Robert J. Graf
Jamie Larsen
John D. Laurie
Pyramiding disease resistance genes in elite winter wheat germplasm for Western Canada
Crop Journal
author_facet André Laroche
Michele Frick
Robert J. Graf
Jamie Larsen
John D. Laurie
author_sort André Laroche
title Pyramiding disease resistance genes in elite winter wheat germplasm for Western Canada
title_short Pyramiding disease resistance genes in elite winter wheat germplasm for Western Canada
title_full Pyramiding disease resistance genes in elite winter wheat germplasm for Western Canada
title_fullStr Pyramiding disease resistance genes in elite winter wheat germplasm for Western Canada
title_full_unstemmed Pyramiding disease resistance genes in elite winter wheat germplasm for Western Canada
title_sort pyramiding disease resistance genes in elite winter wheat germplasm for western canada
publisher KeAi Communications Co., Ltd.
series Crop Journal
issn 2214-5141
publishDate 2019-12-01
description We report on pyramiding different disease resistance genes against fungal pathogens in Canadian winter wheat germplasm based on available DNA markers and gene sequences. Genetic resistance represents a safe, economical and ecological method for protecting plants, growers and the health of consumers. Major diseases of wheat on the Canadian Prairies are common bunt, rusts (leaf, stem and stripe) and Fusarium head blight. Over the years markers for resistance genes against these diseases have been identified and used by the international wheat community. We describe markers that we have used to pyramid different resistance genes and indicate their presence in Canadian winter wheat cultivars issued from the winter wheat breeding program at the Agriculture and Agri-Food Canada, Lethbridge Research and Development Centre, the only winter wheat breeding program in Western Canada actively delivering new varieties for all regions of the Canadian Prairies. The sources of resistance and identities of PCR primers and amplification conditions are indicated to enable the transfer and pyramiding of different resistance (R) genes to breeding lines. We conclude by reviewing new tools for identifying R genes in wheat and indicate how mutagenesis and gene editing can help future efforts to extend the protection offered by known R genes. Keywords: Bunt, Gene pyramiding, Wheat rusts, Marker-assisted selection, Triticum aestivum
url http://www.sciencedirect.com/science/article/pii/S2214514119301230
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