Analysis of zinc oxide nanoparticles binding proteins in rat blood and brain homogenate

Kyu Hwan Shim,1 John Hulme,1 Eun Ho Maeng,2 Meyoung-Kon Kim,3 Seong Soo A An1 1Department of Bionano Technology, Gachon Medical Research Institute, Gachon University, Sungnam-si, Gyeonggi-do, South Korea; 2Department of Analysis, KTR, Kimpo, Gyeonggi-do, South Korea; 3Department of Biochemistry and...

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Main Authors: Shim KH, Hulme J, Maeng EH, Kim MK, An SSA
Format: Article
Language:English
Published: Dove Medical Press 2014-12-01
Series:International Journal of Nanomedicine
Online Access:http://www.dovepress.com/analysis-of-zinc-oxide-nanoparticles-binding-proteins-in-rat-blood-and-peer-reviewed-article-IJN
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spelling doaj-0f6bed58febf4cdbb6313574e29e43632020-11-24T20:43:09ZengDove Medical PressInternational Journal of Nanomedicine1178-20132014-12-012014Supplement 221722419591Analysis of zinc oxide nanoparticles binding proteins in rat blood and brain homogenateShim KHHulme JMaeng EHKim MKAn SSA Kyu Hwan Shim,1 John Hulme,1 Eun Ho Maeng,2 Meyoung-Kon Kim,3 Seong Soo A An1 1Department of Bionano Technology, Gachon Medical Research Institute, Gachon University, Sungnam-si, Gyeonggi-do, South Korea; 2Department of Analysis, KTR, Kimpo, Gyeonggi-do, South Korea; 3Department of Biochemistry and Molecular Biology, Korea University Medical School and College, Seoul, South Korea Abstract: Nanoparticles (NPs) are currently used in chemical, cosmetic, pharmaceutical, and electronic products. Nevertheless, limited safety information is available for many NPs, especially in terms of their interactions with various binding proteins, leading to potential toxic effects. Zinc oxide (ZnO) NPs are included in the formulation of new products, such as adhesives, batteries, ceramics, cosmetics, cement, glass, ointments, paints, pigments, and supplementary foods, resulting in increased human exposures to ZnO. Hence, we investigated the potential ZnO nanotoxic pathways by analyzing the adsorbed proteins, called protein corona, from blood and brain from four ZnO NPs, ZnOSM20(-), ZnOSM20(+), ZnOAE100(-), and ZnOAE100(+), in order to understand their potential mechanisms in vivo. Through this study, liquid chromatography–mass spectroscopy/mass spectroscopy technology was employed to identify all bound proteins. Totals of 52 and 58 plasma proteins were identified as being bound to ZnOSM20(-) and ZnOSM20(+), respectively. For ZnOAE100(-) and ZnOAE100(+), 58 and 44 proteins were bound, respectively. Similar numbers of proteins were adsorbed onto ZnO irrespective of size or surface charge of the nanoparticle. These proteins were further analyzed with ClueGO, a Cytoscape plugin, which provided gene ontology and the biological interaction processes of identified proteins. Interactions between diverse proteins and ZnO nanoparticles could result in an alteration of their functions, conformation, and clearance, eventually affecting many biological processes. Keywords: brain homogenate, nanotoxicity, plasma, protein coronahttp://www.dovepress.com/analysis-of-zinc-oxide-nanoparticles-binding-proteins-in-rat-blood-and-peer-reviewed-article-IJN
collection DOAJ
language English
format Article
sources DOAJ
author Shim KH
Hulme J
Maeng EH
Kim MK
An SSA
spellingShingle Shim KH
Hulme J
Maeng EH
Kim MK
An SSA
Analysis of zinc oxide nanoparticles binding proteins in rat blood and brain homogenate
International Journal of Nanomedicine
author_facet Shim KH
Hulme J
Maeng EH
Kim MK
An SSA
author_sort Shim KH
title Analysis of zinc oxide nanoparticles binding proteins in rat blood and brain homogenate
title_short Analysis of zinc oxide nanoparticles binding proteins in rat blood and brain homogenate
title_full Analysis of zinc oxide nanoparticles binding proteins in rat blood and brain homogenate
title_fullStr Analysis of zinc oxide nanoparticles binding proteins in rat blood and brain homogenate
title_full_unstemmed Analysis of zinc oxide nanoparticles binding proteins in rat blood and brain homogenate
title_sort analysis of zinc oxide nanoparticles binding proteins in rat blood and brain homogenate
publisher Dove Medical Press
series International Journal of Nanomedicine
issn 1178-2013
publishDate 2014-12-01
description Kyu Hwan Shim,1 John Hulme,1 Eun Ho Maeng,2 Meyoung-Kon Kim,3 Seong Soo A An1 1Department of Bionano Technology, Gachon Medical Research Institute, Gachon University, Sungnam-si, Gyeonggi-do, South Korea; 2Department of Analysis, KTR, Kimpo, Gyeonggi-do, South Korea; 3Department of Biochemistry and Molecular Biology, Korea University Medical School and College, Seoul, South Korea Abstract: Nanoparticles (NPs) are currently used in chemical, cosmetic, pharmaceutical, and electronic products. Nevertheless, limited safety information is available for many NPs, especially in terms of their interactions with various binding proteins, leading to potential toxic effects. Zinc oxide (ZnO) NPs are included in the formulation of new products, such as adhesives, batteries, ceramics, cosmetics, cement, glass, ointments, paints, pigments, and supplementary foods, resulting in increased human exposures to ZnO. Hence, we investigated the potential ZnO nanotoxic pathways by analyzing the adsorbed proteins, called protein corona, from blood and brain from four ZnO NPs, ZnOSM20(-), ZnOSM20(+), ZnOAE100(-), and ZnOAE100(+), in order to understand their potential mechanisms in vivo. Through this study, liquid chromatography–mass spectroscopy/mass spectroscopy technology was employed to identify all bound proteins. Totals of 52 and 58 plasma proteins were identified as being bound to ZnOSM20(-) and ZnOSM20(+), respectively. For ZnOAE100(-) and ZnOAE100(+), 58 and 44 proteins were bound, respectively. Similar numbers of proteins were adsorbed onto ZnO irrespective of size or surface charge of the nanoparticle. These proteins were further analyzed with ClueGO, a Cytoscape plugin, which provided gene ontology and the biological interaction processes of identified proteins. Interactions between diverse proteins and ZnO nanoparticles could result in an alteration of their functions, conformation, and clearance, eventually affecting many biological processes. Keywords: brain homogenate, nanotoxicity, plasma, protein corona
url http://www.dovepress.com/analysis-of-zinc-oxide-nanoparticles-binding-proteins-in-rat-blood-and-peer-reviewed-article-IJN
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