Optimum Conditions for Uricase Enzyme Production by Gliomastix gueg

• Main Authors: Atalla, M. M., Farag, M. M., Eman, R. H., Abd-El-Lataif, M. S., Nehad, E. A.
• Format: Article
• Language: English
• Published: Malaysian Society for Microbiology 2009-01-01
• Series: Malaysian Journal of Microbiology
• Subjects: Gliomastix gueg; Uricase production; Physiological conditions
• Online Access: http://web.usm.my/mjm/issues/vol5no1/research8.pdf

Nineteen strains of microorganisms were screened for uricase production. Gliomastix gueg was recognized to produce high levels of the enzyme. The optimum fermentation conditions for uricase production by Gliomastix gueg were examined. Results showed that uric acid medium was the most favorable one, the optimum temperature was at 30ºC, and incubation period required for maximum production was 8 days with aeration level at 150 rpm and at pH 8.0. Sucrose proved to be the best carbon source, uric acid was found to be the best nitrogen source. Both, dipotassium hydrogen phosphate and ferrous chloride as well as some vitamins gave the highest amount of uricase by Gliomastix gueg.