The molecular changing mechanism of Ampicillin-Sulbactam resistant Staphylococcus aureus towards Methicillin resistant Staphylococcus aureus

• Main Author: Mieke Hemiawati Satari
• Format: Article
• Language: English
• Published: Universitas Airlangga 2005-12-01
• Series: Dental Journal: Majalah Kedokteran Gigi
• Subjects: S.aureus; betalactamase gene; PBP 2a gene
• Online Access: http://e-journal.unair.ac.id/index.php/MKG/article/view/1118

<span style="font-family: Tribune,Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>The aim of this study was to determine the molecular changing of S.aureus, which is resistant to Ampicillin-Sulbactam and then </em><span style="font-family: Tribune,Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>become resistant to Methicillin as a result of improper dosage. The study was conducted by isolating Ampicillin-Sulbactam resistant </em><span style="font-family: Tribune,Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>and Methicillin Resistant S.aureus (MRSA), afterwards an amplification process was performed by PCR (Polymerase Chain Reaction.) </em><span style="font-family: Tribune,Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>to isolate the betalactamase enzyme regulator and PBP 2a genes. The result of this research showed that there were a deletion of </em><span style="font-family: Tribune,Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>few amino acids from the regulator gene, and a suspicion that the DNA sequence had been substituted from PBP 2 gene into PBP 2a </em><span style="font-family: Tribune,Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>(gen mec). This process had formed MRSA.</em></span></span></span></span></span><br style="font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;" /></span>