Evaluation of 16S rDNA Heart Tissue PCR as a Complement to Blood Cultures for the Routine Etiological Diagnosis of Infective Endocarditis

• Main Authors: Raquel Rodríguez-García, María Ángeles Rodríguez-Esteban, Jonathan Fernández-Suárez, Ana Morilla, Enrique García-Carús, Mauricio Telenti, Carlos Morales, Guillermo Muñiz Albaiceta, Javier Fernández
• Format: Article
• Language: English
• Published: MDPI AG 2021-07-01
• Series: Diagnostics
• Subjects: infective endocarditis; 16S rDNA PCR; blood culture; valve heart culture; molecular diagnosis
• Online Access: https://www.mdpi.com/2075-4418/11/8/1372

Identification of the causative pathogen is required to optimize the effective therapy in infective endocarditis (IE). The aim of this study was to assess a 16S rDNA PCR to identify bacteria from heart valve tissues and to evaluate its usefulness as a complement to blood and removed valves cultures. A total of 266 patients diagnosed with IE from January 2015 to December 2019 were evaluated. Results between 16S rDNA PCR from heart valve tissues were compared with microbiological cultures. Blood cultures were positive in 83.5% of patients diagnosed with IE, while 39.6% and 71.8% of the evaluated heart valve samples were positive by culture and 16S rDNA PCR, respectively. For 32 (12%) patients, 16S rDNA tissue PCR provided valuable information supporting the results of blood cultures in the case of bacteria characteristic from the skin microbiota. Additionally, a microorganism was identified by using 16S rDNA PCR in 36% of blood culture-negative cases. The present study reveals that molecular diagnosis using 16S rDNA tissue PCR provides complementary information for the diagnosis of IE, and it should be recommended in surgical endocarditis, especially when blood cultures are negative.