Effects of nonspecific resistance in barley genotypes obtained by cell selection

An effective way to increase the genetic diversity of plants and create sources of resistance to edaphic stressors is the selection of cells under selective in vitro conditions based on somaclonal variation. The use of somaclones (regenerant forms) of barley with resistance to increased acidity, ion...

Full description

Bibliographic Details
Main Authors: O. N. Shupletsova, S. Yu. Ogorodnikova, Ya. I. Nazarova
Format: Article
Language:English
Published: N.I. Vavilov All-Russian Institute of Plant Genetic Resources 2020-12-01
Series:Труды по прикладной ботанике, генетике и селекции
Subjects:
Online Access:https://elpub.vir.nw.ru/jour/article/view/823
id doaj-0ee83614006d47378d17390b7a51d476
record_format Article
spelling doaj-0ee83614006d47378d17390b7a51d4762021-07-05T03:33:10ZengN.I. Vavilov All-Russian Institute of Plant Genetic ResourcesТруды по прикладной ботанике, генетике и селекции2227-88342619-09822020-12-01181419219910.30901/2227-8834-2020-4-192-199489Effects of nonspecific resistance in barley genotypes obtained by cell selectionO. N. Shupletsova0S. Yu. Ogorodnikova1Ya. I. Nazarova2N.V. Rudnitsky Federal Agricultural Science Center of the North-EastInstitute of Biology, Komi Science Center, Ural Branch of the RASN.V. Rudnitsky Federal Agricultural Science Center of the North-EastAn effective way to increase the genetic diversity of plants and create sources of resistance to edaphic stressors is the selection of cells under selective in vitro conditions based on somaclonal variation. The use of somaclones (regenerant forms) of barley with resistance to increased acidity, ionic toxicity of aluminum, heavy metals and drought is promising in the northeast of the Non-Black-Soil Zone of Russia. Development of regenerants with integrated resistance to soil stressors due to specific and nonspecific mechanisms is a relevant trend.The target research material was a hybrid combination of spring barley (Luly × Conrad) × 2867-80 and its regenerant forms obtained as a result of cell selection with stressors of various nature. Plants were grown under ordinary soil conditions and on provocative backgrounds with aluminum and cadmium. The productive traits of plants, the symptoms of oxidative stress, and the environment-forming activity of the root system were evaluated.In regenerated plants obtained in callus culture on selective in vitro media with aluminum or water deficiency, an increased ability of the root system to alkalize the medium in the rhizosphere zone (by 0.2–0.5 pH units) when grown on alumina soil was revealed. Against stressful soil backgrounds, regenerative lines induced on in vitro media with cadmium or aluminum were observed to have a low manifestation of oxidative stress symptoms, tested by the intensity of lipid peroxidation and the content of photosynthetic pigments in the leaves, which provided these genotypes with higher seed productivity (1.5–3.6 times in the number of grains, and 1.5– 3.0 times in the grain weight per plant) and adaptive advantages compared to the original genotype and regenerants induced on an in vitro medium with water deficiency. It is concluded that complex resistance to soil aluminum and cadmium toxicity is formed in barley regenerants obtained in the process of cell selection on in vitro media with any of these metals.https://elpub.vir.nw.ru/jour/article/view/823stressin vitro selectionaluminumcadmiumdroughtinitial genotyperegenerantprovocative backgroundoxidative stressenvironment-forming activityproductive traits
collection DOAJ
language English
format Article
sources DOAJ
author O. N. Shupletsova
S. Yu. Ogorodnikova
Ya. I. Nazarova
spellingShingle O. N. Shupletsova
S. Yu. Ogorodnikova
Ya. I. Nazarova
Effects of nonspecific resistance in barley genotypes obtained by cell selection
Труды по прикладной ботанике, генетике и селекции
stress
in vitro selection
aluminum
cadmium
drought
initial genotype
regenerant
provocative background
oxidative stress
environment-forming activity
productive traits
author_facet O. N. Shupletsova
S. Yu. Ogorodnikova
Ya. I. Nazarova
author_sort O. N. Shupletsova
title Effects of nonspecific resistance in barley genotypes obtained by cell selection
title_short Effects of nonspecific resistance in barley genotypes obtained by cell selection
title_full Effects of nonspecific resistance in barley genotypes obtained by cell selection
title_fullStr Effects of nonspecific resistance in barley genotypes obtained by cell selection
title_full_unstemmed Effects of nonspecific resistance in barley genotypes obtained by cell selection
title_sort effects of nonspecific resistance in barley genotypes obtained by cell selection
publisher N.I. Vavilov All-Russian Institute of Plant Genetic Resources
series Труды по прикладной ботанике, генетике и селекции
issn 2227-8834
2619-0982
publishDate 2020-12-01
description An effective way to increase the genetic diversity of plants and create sources of resistance to edaphic stressors is the selection of cells under selective in vitro conditions based on somaclonal variation. The use of somaclones (regenerant forms) of barley with resistance to increased acidity, ionic toxicity of aluminum, heavy metals and drought is promising in the northeast of the Non-Black-Soil Zone of Russia. Development of regenerants with integrated resistance to soil stressors due to specific and nonspecific mechanisms is a relevant trend.The target research material was a hybrid combination of spring barley (Luly × Conrad) × 2867-80 and its regenerant forms obtained as a result of cell selection with stressors of various nature. Plants were grown under ordinary soil conditions and on provocative backgrounds with aluminum and cadmium. The productive traits of plants, the symptoms of oxidative stress, and the environment-forming activity of the root system were evaluated.In regenerated plants obtained in callus culture on selective in vitro media with aluminum or water deficiency, an increased ability of the root system to alkalize the medium in the rhizosphere zone (by 0.2–0.5 pH units) when grown on alumina soil was revealed. Against stressful soil backgrounds, regenerative lines induced on in vitro media with cadmium or aluminum were observed to have a low manifestation of oxidative stress symptoms, tested by the intensity of lipid peroxidation and the content of photosynthetic pigments in the leaves, which provided these genotypes with higher seed productivity (1.5–3.6 times in the number of grains, and 1.5– 3.0 times in the grain weight per plant) and adaptive advantages compared to the original genotype and regenerants induced on an in vitro medium with water deficiency. It is concluded that complex resistance to soil aluminum and cadmium toxicity is formed in barley regenerants obtained in the process of cell selection on in vitro media with any of these metals.
topic stress
in vitro selection
aluminum
cadmium
drought
initial genotype
regenerant
provocative background
oxidative stress
environment-forming activity
productive traits
url https://elpub.vir.nw.ru/jour/article/view/823
work_keys_str_mv AT onshupletsova effectsofnonspecificresistanceinbarleygenotypesobtainedbycellselection
AT syuogorodnikova effectsofnonspecificresistanceinbarleygenotypesobtainedbycellselection
AT yainazarova effectsofnonspecificresistanceinbarleygenotypesobtainedbycellselection
_version_ 1721319117361774592