Single cell transcriptomics identifies a signaling network coordinating endoderm and mesoderm diversification during foregut organogenesis

The fetal murine foregut develops into visceral organs via interactions between the mesoderm and endoderm, but how is unclear. Here, the authors use single cell RNAseq to show a diversity in organ specific splanchnic mesoderm cell-types, infer a signalling network governing organogenesis and use thi...

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Main Authors:	Lu Han, Praneet Chaturvedi, Keishi Kishimoto, Hiroyuki Koike, Talia Nasr, Kentaro Iwasawa, Kirsten Giesbrecht, Phillip C. Witcher, Alexandra Eicher, Lauren Haines, Yarim Lee, John M. Shannon, Mitsuru Morimoto, James M. Wells, Takanori Takebe, Aaron M. Zorn
Format:	Article
Language:	English
Published:	Nature Publishing Group 2020-08-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-020-17968-x

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spelling	doaj-0ee51be150a74c26a5360813006411972021-08-29T11:38:44ZengNature Publishing GroupNature Communications2041-17232020-08-0111111610.1038/s41467-020-17968-xSingle cell transcriptomics identifies a signaling network coordinating endoderm and mesoderm diversification during foregut organogenesisLu Han0Praneet Chaturvedi1Keishi Kishimoto2Hiroyuki Koike3Talia Nasr4Kentaro Iwasawa5Kirsten Giesbrecht6Phillip C. Witcher7Alexandra Eicher8Lauren Haines9Yarim Lee10John M. Shannon11Mitsuru Morimoto12James M. Wells13Takanori Takebe14Aaron M. Zorn15Center for Stem Cell and Organoid Medicine (CuSTOM), Perinatal Institute, Division of Developmental Biology, Cincinnati Children’s Hospital, Department of Pediatrics, University of Cincinnati, College of MedicineCenter for Stem Cell and Organoid Medicine (CuSTOM), Perinatal Institute, Division of Developmental Biology, Cincinnati Children’s Hospital, Department of Pediatrics, University of Cincinnati, College of MedicineCenter for Stem Cell and Organoid Medicine (CuSTOM), Perinatal Institute, Division of Developmental Biology, Cincinnati Children’s Hospital, Department of Pediatrics, University of Cincinnati, College of MedicineCuSTOM, Division of Gastroenterology, Cincinnati Children’s Hospital, Department of Pediatrics, University of Cincinnati, College of MedicineCenter for Stem Cell and Organoid Medicine (CuSTOM), Perinatal Institute, Division of Developmental Biology, Cincinnati Children’s Hospital, Department of Pediatrics, University of Cincinnati, College of MedicineCuSTOM, Division of Gastroenterology, Cincinnati Children’s Hospital, Department of Pediatrics, University of Cincinnati, College of MedicineCuSTOM, Division of Gastroenterology, Cincinnati Children’s Hospital, Department of Pediatrics, University of Cincinnati, College of MedicineCenter for Stem Cell and Organoid Medicine (CuSTOM), Perinatal Institute, Division of Developmental Biology, Cincinnati Children’s Hospital, Department of Pediatrics, University of Cincinnati, College of MedicineCenter for Stem Cell and Organoid Medicine (CuSTOM), Perinatal Institute, Division of Developmental Biology, Cincinnati Children’s Hospital, Department of Pediatrics, University of Cincinnati, College of MedicineCenter for Stem Cell and Organoid Medicine (CuSTOM), Perinatal Institute, Division of Developmental Biology, Cincinnati Children’s Hospital, Department of Pediatrics, University of Cincinnati, College of MedicineCenter for Stem Cell and Organoid Medicine (CuSTOM), Perinatal Institute, Division of Developmental Biology, Cincinnati Children’s Hospital, Department of Pediatrics, University of Cincinnati, College of MedicineDivision of Pulmonary Biology, Cincinnati Children’s Hospital, Department of Pediatrics, University of Cincinnati, College of MedicineLaboratory for Lung Development, RIKEN Center for Biosystems Dynamics Research (BDR)Center for Stem Cell and Organoid Medicine (CuSTOM), Perinatal Institute, Division of Developmental Biology, Cincinnati Children’s Hospital, Department of Pediatrics, University of Cincinnati, College of MedicineCuSTOM, Division of Gastroenterology, Cincinnati Children’s Hospital, Department of Pediatrics, University of Cincinnati, College of MedicineCenter for Stem Cell and Organoid Medicine (CuSTOM), Perinatal Institute, Division of Developmental Biology, Cincinnati Children’s Hospital, Department of Pediatrics, University of Cincinnati, College of MedicineThe fetal murine foregut develops into visceral organs via interactions between the mesoderm and endoderm, but how is unclear. Here, the authors use single cell RNAseq to show a diversity in organ specific splanchnic mesoderm cell-types, infer a signalling network governing organogenesis and use this to differentiate human pluripotent stem cells.https://doi.org/10.1038/s41467-020-17968-x
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Lu Han Praneet Chaturvedi Keishi Kishimoto Hiroyuki Koike Talia Nasr Kentaro Iwasawa Kirsten Giesbrecht Phillip C. Witcher Alexandra Eicher Lauren Haines Yarim Lee John M. Shannon Mitsuru Morimoto James M. Wells Takanori Takebe Aaron M. Zorn
spellingShingle	Lu Han Praneet Chaturvedi Keishi Kishimoto Hiroyuki Koike Talia Nasr Kentaro Iwasawa Kirsten Giesbrecht Phillip C. Witcher Alexandra Eicher Lauren Haines Yarim Lee John M. Shannon Mitsuru Morimoto James M. Wells Takanori Takebe Aaron M. Zorn Single cell transcriptomics identifies a signaling network coordinating endoderm and mesoderm diversification during foregut organogenesis Nature Communications
author_facet	Lu Han Praneet Chaturvedi Keishi Kishimoto Hiroyuki Koike Talia Nasr Kentaro Iwasawa Kirsten Giesbrecht Phillip C. Witcher Alexandra Eicher Lauren Haines Yarim Lee John M. Shannon Mitsuru Morimoto James M. Wells Takanori Takebe Aaron M. Zorn
author_sort	Lu Han
title	Single cell transcriptomics identifies a signaling network coordinating endoderm and mesoderm diversification during foregut organogenesis
title_short	Single cell transcriptomics identifies a signaling network coordinating endoderm and mesoderm diversification during foregut organogenesis
title_full	Single cell transcriptomics identifies a signaling network coordinating endoderm and mesoderm diversification during foregut organogenesis
title_fullStr	Single cell transcriptomics identifies a signaling network coordinating endoderm and mesoderm diversification during foregut organogenesis
title_full_unstemmed	Single cell transcriptomics identifies a signaling network coordinating endoderm and mesoderm diversification during foregut organogenesis
title_sort	single cell transcriptomics identifies a signaling network coordinating endoderm and mesoderm diversification during foregut organogenesis
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2020-08-01
description	The fetal murine foregut develops into visceral organs via interactions between the mesoderm and endoderm, but how is unclear. Here, the authors use single cell RNAseq to show a diversity in organ specific splanchnic mesoderm cell-types, infer a signalling network governing organogenesis and use this to differentiate human pluripotent stem cells.
url	https://doi.org/10.1038/s41467-020-17968-x
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Single cell transcriptomics identifies a signaling network coordinating endoderm and mesoderm diversification during foregut organogenesis

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