Community Assembly of Endophytic Fungi in Ectomycorrhizae of Betulaceae Plants at a Regional Scale

The interaction between aboveground and belowground biotic communities drives community assembly of plants and soil microbiota. As an important component of belowground microorganisms, root-associated fungi play pivotal roles in biodiversity maintenance and community assembly of host plants. The Bet...

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Main Authors: Yong-Long Wang, Cheng Gao, Liang Chen, Niu-Niu Ji, Bin-Wei Wu, Peng-Peng Lü, Xing-Chun Li, Xin Qian, Pulak Maitra, Busayo Joshua Babalola, Yong Zheng, Liang-Dong Guo
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-01-01
Series:Frontiers in Microbiology
Subjects:
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2019.03105/full
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language English
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author Yong-Long Wang
Yong-Long Wang
Cheng Gao
Liang Chen
Niu-Niu Ji
Niu-Niu Ji
Bin-Wei Wu
Bin-Wei Wu
Peng-Peng Lü
Peng-Peng Lü
Xing-Chun Li
Xin Qian
Xin Qian
Pulak Maitra
Pulak Maitra
Busayo Joshua Babalola
Busayo Joshua Babalola
Yong Zheng
Yong Zheng
Liang-Dong Guo
Liang-Dong Guo
spellingShingle Yong-Long Wang
Yong-Long Wang
Cheng Gao
Liang Chen
Niu-Niu Ji
Niu-Niu Ji
Bin-Wei Wu
Bin-Wei Wu
Peng-Peng Lü
Peng-Peng Lü
Xing-Chun Li
Xin Qian
Xin Qian
Pulak Maitra
Pulak Maitra
Busayo Joshua Babalola
Busayo Joshua Babalola
Yong Zheng
Yong Zheng
Liang-Dong Guo
Liang-Dong Guo
Community Assembly of Endophytic Fungi in Ectomycorrhizae of Betulaceae Plants at a Regional Scale
Frontiers in Microbiology
Betulaceae
dispersal limitation
endophytic fungi
environmental filtering
host phylogeny
author_facet Yong-Long Wang
Yong-Long Wang
Cheng Gao
Liang Chen
Niu-Niu Ji
Niu-Niu Ji
Bin-Wei Wu
Bin-Wei Wu
Peng-Peng Lü
Peng-Peng Lü
Xing-Chun Li
Xin Qian
Xin Qian
Pulak Maitra
Pulak Maitra
Busayo Joshua Babalola
Busayo Joshua Babalola
Yong Zheng
Yong Zheng
Liang-Dong Guo
Liang-Dong Guo
author_sort Yong-Long Wang
title Community Assembly of Endophytic Fungi in Ectomycorrhizae of Betulaceae Plants at a Regional Scale
title_short Community Assembly of Endophytic Fungi in Ectomycorrhizae of Betulaceae Plants at a Regional Scale
title_full Community Assembly of Endophytic Fungi in Ectomycorrhizae of Betulaceae Plants at a Regional Scale
title_fullStr Community Assembly of Endophytic Fungi in Ectomycorrhizae of Betulaceae Plants at a Regional Scale
title_full_unstemmed Community Assembly of Endophytic Fungi in Ectomycorrhizae of Betulaceae Plants at a Regional Scale
title_sort community assembly of endophytic fungi in ectomycorrhizae of betulaceae plants at a regional scale
publisher Frontiers Media S.A.
series Frontiers in Microbiology
issn 1664-302X
publishDate 2020-01-01
description The interaction between aboveground and belowground biotic communities drives community assembly of plants and soil microbiota. As an important component of belowground microorganisms, root-associated fungi play pivotal roles in biodiversity maintenance and community assembly of host plants. The Betulaceae plants form ectomycorrhizae with soil fungi and widely distribute in various ecosystems. However, the community assembly of endophytic fungi in ectomycorrhizae is less investigated at a large spatial scale. Here, we examined the endophytic fungal communities in ectomycorrhizae of 22 species in four genera belonging to Betulaceae in Chinese forest ecosystems, using Illumina Miseq sequencing of internal transcribed spacer 2 amplicons. The relative contribution of host phylogeny, climate and soil (environmental filtering) and geographic distance (dispersal limitation) on endophytic fungal community was disentangled. In total, 2,106 endophytic fungal operational taxonomic units (OTUs) were obtained at a 97% sequence similarity level, dominated by Leotiomycetes, Agaricomycetes, Eurotiomycetes, and Sordariomycetes. The endophytic fungal OTU richness was significantly related with host phylogeny, geographic distance, soil and climate. The endophytic fungal community composition was significantly affected by host phylogeny (19.5% of variation explained in fungal community), geographic distance (11.2%), soil (6.1%), and climate (1.4%). This finding suggests that environmental filtering by plant and abiotic variables coupled with dispersal limitation linked to geographic distance determines endophytic fungal community assembly in ectomycorrhizae of Betulaceae plants, with host phylogeny being a stronger determinant than other predictor variables at the regional scale.
topic Betulaceae
dispersal limitation
endophytic fungi
environmental filtering
host phylogeny
url https://www.frontiersin.org/article/10.3389/fmicb.2019.03105/full
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spelling doaj-0eb3713ff2da4ba89e5829bf4a554d092020-11-25T02:36:24ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-01-011010.3389/fmicb.2019.03105473297Community Assembly of Endophytic Fungi in Ectomycorrhizae of Betulaceae Plants at a Regional ScaleYong-Long Wang0Yong-Long Wang1Cheng Gao2Liang Chen3Niu-Niu Ji4Niu-Niu Ji5Bin-Wei Wu6Bin-Wei Wu7Peng-Peng Lü8Peng-Peng Lü9Xing-Chun Li10Xin Qian11Xin Qian12Pulak Maitra13Pulak Maitra14Busayo Joshua Babalola15Busayo Joshua Babalola16Yong Zheng17Yong Zheng18Liang-Dong Guo19Liang-Dong Guo20State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, ChinaState Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaCollege of Geographical Science, Fujian Normal University, Fuzhou, ChinaState Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing, ChinaThe interaction between aboveground and belowground biotic communities drives community assembly of plants and soil microbiota. As an important component of belowground microorganisms, root-associated fungi play pivotal roles in biodiversity maintenance and community assembly of host plants. The Betulaceae plants form ectomycorrhizae with soil fungi and widely distribute in various ecosystems. However, the community assembly of endophytic fungi in ectomycorrhizae is less investigated at a large spatial scale. Here, we examined the endophytic fungal communities in ectomycorrhizae of 22 species in four genera belonging to Betulaceae in Chinese forest ecosystems, using Illumina Miseq sequencing of internal transcribed spacer 2 amplicons. The relative contribution of host phylogeny, climate and soil (environmental filtering) and geographic distance (dispersal limitation) on endophytic fungal community was disentangled. In total, 2,106 endophytic fungal operational taxonomic units (OTUs) were obtained at a 97% sequence similarity level, dominated by Leotiomycetes, Agaricomycetes, Eurotiomycetes, and Sordariomycetes. The endophytic fungal OTU richness was significantly related with host phylogeny, geographic distance, soil and climate. The endophytic fungal community composition was significantly affected by host phylogeny (19.5% of variation explained in fungal community), geographic distance (11.2%), soil (6.1%), and climate (1.4%). This finding suggests that environmental filtering by plant and abiotic variables coupled with dispersal limitation linked to geographic distance determines endophytic fungal community assembly in ectomycorrhizae of Betulaceae plants, with host phylogeny being a stronger determinant than other predictor variables at the regional scale.https://www.frontiersin.org/article/10.3389/fmicb.2019.03105/fullBetulaceaedispersal limitationendophytic fungienvironmental filteringhost phylogeny