A Capillary-Perfused, Nanocalorimeter Platform for Thermometric Enzyme-Linked Immunosorbent Assay with Femtomole Sensitivity

Enzyme-catalyzed chemical reactions produce heat. We developed an enclosed, capillary-perfused nanocalorimeter platform for thermometric enzyme-linked immunosorbent assay (TELISA). We used catalase as enzymes to model the thermal characteristics of the micromachined calorimeter. Model-assisted signa...

Full description

Bibliographic Details
Main Authors: Evan Kazura, Ray Mernaugh, Franz Baudenbacher
Format: Article
Language:English
Published: MDPI AG 2020-06-01
Series:Biosensors
Subjects:
Online Access:https://www.mdpi.com/2079-6374/10/6/71