Study on the Preservation with CO (PCO = 200–2,000 hPa), Resuscitation, and Heterotopic Transplantation of an Isolated Rat Heart

Study on the Preservation with CO (PCO = 200–2,000 hPa), Resuscitation, and Heterotopic Transplantation of an Isolated Rat Heart

In this experiment, CO was used as a gas mixture in a reversible relationship with O 2 . CO was added in a gas form mixed with O 2 . An isolated donor rat heart was obtained, exposed to a gas mixture such as PO 2 = 1,800 hPa and PCO = 200 hPa, and PO 2 = 1,000 hPa and PCO = 1,000 hPa in a 2 ATA high...

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Main Authors: Yu Yoshida, Naoyuki Hatayama, Kunihiro Seki
Format: Article
Language:English
Published: SAGE Publishing 2009-05-01
Series:Cell Transplantation
Online Access:https://doi.org/10.1177/096368970901805-608
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spelling doaj-0e85375c90014556b3d87a4fc0334bf42020-11-25T03:16:20ZengSAGE PublishingCell Transplantation0963-68971555-38922009-05-011810.1177/096368970901805-608Study on the Preservation with CO (PCO = 200–2,000 hPa), Resuscitation, and Heterotopic Transplantation of an Isolated Rat HeartYu Yoshida0Naoyuki Hatayama1Kunihiro Seki2Kanagawa University Faculty of Science, Hiratsukashi, 259-1293 JapanKanagawa University Faculty of Science, Hiratsukashi, 259-1293 JapanKanagawa University Faculty of Science, Hiratsukashi, 259-1293 JapanIn this experiment, CO was used as a gas mixture in a reversible relationship with O 2 . CO was added in a gas form mixed with O 2 . An isolated donor rat heart was obtained, exposed to a gas mixture such as PO 2 = 1,800 hPa and PCO = 200 hPa, and PO 2 = 1,000 hPa and PCO = 1,000 hPa in a 2 ATA high-pressure chamber and preserved in a refrigerator at 4°C. This report demonstrates that significant reproducibility has been verified. The heart was removed from the refrigerator 24 h later and heterotopic heart transplantation was performed in the right neck of a recipient rat and the pulsating of the transplanted heart was detected by an electrocardiogram.https://doi.org/10.1177/096368970901805-608
collection DOAJ
language English
format Article
sources DOAJ
author Yu Yoshida
Naoyuki Hatayama
Kunihiro Seki
spellingShingle Yu Yoshida
Naoyuki Hatayama
Kunihiro Seki
Study on the Preservation with CO (PCO = 200–2,000 hPa), Resuscitation, and Heterotopic Transplantation of an Isolated Rat Heart
Cell Transplantation
author_facet Yu Yoshida
Naoyuki Hatayama
Kunihiro Seki
author_sort Yu Yoshida
title Study on the Preservation with CO (PCO = 200–2,000 hPa), Resuscitation, and Heterotopic Transplantation of an Isolated Rat Heart
title_short Study on the Preservation with CO (PCO = 200–2,000 hPa), Resuscitation, and Heterotopic Transplantation of an Isolated Rat Heart
title_full Study on the Preservation with CO (PCO = 200–2,000 hPa), Resuscitation, and Heterotopic Transplantation of an Isolated Rat Heart
title_fullStr Study on the Preservation with CO (PCO = 200–2,000 hPa), Resuscitation, and Heterotopic Transplantation of an Isolated Rat Heart
title_full_unstemmed Study on the Preservation with CO (PCO = 200–2,000 hPa), Resuscitation, and Heterotopic Transplantation of an Isolated Rat Heart
title_sort study on the preservation with co (pco = 200–2,000 hpa), resuscitation, and heterotopic transplantation of an isolated rat heart
publisher SAGE Publishing
series Cell Transplantation
issn 0963-6897
1555-3892
publishDate 2009-05-01
description In this experiment, CO was used as a gas mixture in a reversible relationship with O 2 . CO was added in a gas form mixed with O 2 . An isolated donor rat heart was obtained, exposed to a gas mixture such as PO 2 = 1,800 hPa and PCO = 200 hPa, and PO 2 = 1,000 hPa and PCO = 1,000 hPa in a 2 ATA high-pressure chamber and preserved in a refrigerator at 4°C. This report demonstrates that significant reproducibility has been verified. The heart was removed from the refrigerator 24 h later and heterotopic heart transplantation was performed in the right neck of a recipient rat and the pulsating of the transplanted heart was detected by an electrocardiogram.
url https://doi.org/10.1177/096368970901805-608
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AT naoyukihatayama studyonthepreservationwithcopco2002000hparesuscitationandheterotopictransplantationofanisolatedratheart
AT kunihiroseki studyonthepreservationwithcopco2002000hparesuscitationandheterotopictransplantationofanisolatedratheart
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