Detection of telomerase activity in Plasmodium falciparum using a nonradioactive method
A simple, quick and sensitive method was used to detect telomerase activity in Plasmodium falciparum. The telomeric repeat amplification protocol (TRAP assay) was modified using electrophoresis and staining with SYBR-green I to detect telomerase activity in a range of 10² to 10(7) parasites. This mi...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Instituto Oswaldo Cruz, Ministério da Saúde
2003-01-01
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| Series: | Memórias do Instituto Oswaldo Cruz. |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000500018 |
| Summary: | A simple, quick and sensitive method was used to detect telomerase activity in Plasmodium falciparum. The telomeric repeat amplification protocol (TRAP assay) was modified using electrophoresis and staining with SYBR-green I to detect telomerase activity in a range of 10² to 10(7) parasites. This might be a useful way to ascertain telomerase activity in different types of nontumor cells. |
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| ISSN: | 0074-0276 1678-8060 |