Transmembrane Potential of Red Blood Cells Under Low Ionic Strength Conditions

• Main Authors: Daniel Moersdorf, Stephane Egee, Claudia Hahn, Benjamin Hanf, Clive Ellory, Serge Thomas, Ingolf Bernhardt
• Format: Article
• Language: English
• Published: Cell Physiol Biochem Press GmbH & Co KG 2013-06-01
• Series: Cellular Physiology and Biochemistry
• Subjects: DiBAC4(3); Flow cytometry; Red blood cells; Transmembrane potential; Low ionic strength; CCCP
• Online Access: http://www.karger.com/Article/FullText/350105

Background/Aims: In a variety of investigations described in the literature it was not clear to what extent the transmembrane potential red blood cells (RBCs) was changed after the cells have been transferred into low ionic strength (LIS) solutions. Another open question was to find out how fast the transmembrane potential of RBCs in LIS solution will change and which final new equilibrium value will be reached. Methods: The transmembrane potential of human and bovine RBCs was investigated using the potential-sensitive fluorescent dye DIBAC4(3) (bis(1,3-dibutylbarbituric acid) trimethine oxonol) as well as the CCCP (carbonylcyanide-m-chlorophenylhydrazone) method. Results: Under physiological conditions the transmembrane potential was about -10 mV in agreement with literature data. However, when the RBCs were transferred into an isosmotic low ionic strength medium containing sucrose the transmembrane potential increased to +73 mV and +81 mV for human and bovine RBCs, respectively. In case of human RBCs it continuously decreased reaching finally an equilibrium state of -10 mV again after 30 - 60 min. For bovine RBCs the transmembrane potential declined more slowly reaching a value of +72 mV after 30 min. Conclusions: Investigations of parameters of RBCs depending on transmembrane potential cannot be performed with human RBCs in LIS media.