| Summary: | 【Abstract】Objective: To explore the protective
effects of two types of ischemic postconditioning (IP) on
intestinal mucosa barrier in rabbits with crush injury of the
hind limb.
Methods: This study was conducted between August
and December 2008 in the Department of Trauma Surgery,
Daping Hospital, Third Military Medical University,
Chongqing, China. The model of crush injury to the hind
limb of rabbits was firstly developed by a 25 kg object with
the right hind limbs fixed by wooden splints, and then two
types of IP were established, including occluding/opening
the common iliac artery and vein alternatively (traditional
IP, IP A) and binding/loosening the proximum of the injured
hind limb alternatively (modified IP, IP B). Thirty-six male
New Zealand white rabbits were randomly divided into three
groups: IP A group, IP B group and control group, with 12
rabbits in each group. The serum levels of diamine oxidase
(DAO) and intestinal fatty acid-binding protein (I-FABP)
were detected at 2, 6, 12 and 24 hours after injury. Pathological
changes of ileum were examined at 24 hours after injury.
Results: The serum levels of I-FABP at 2, 6, 12 and 24
hours after injury in both IP A and IP B groups had a significant
decrease, compared with control group. DAO levels
also showed the same change trend at 2 and 6 hours after
injury, but showed no significant difference between two IP
groups. No difference in pathological changes of ileum was
found among the three groups.
Conclusions: IP can protect intestinal mucosa barrier
function on the model of hind limb crush injury in rabbits.
Meanwhile the modified IP B shows the same protection as
the traditional IP A, and is worth applying in clinic.
Key words: Ischemic postconditioning; Crush
syndrome; Intestinal mucosa
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