Hyperspectral imaging for monitoring oxygen saturation levels during normothermic kidney perfusion
The development of improved preservation techniques and the reliable assessment of donor grafts are main fields of research in transplantation medicine. Normothermic machine perfusion (NMP) is a promising alternative to static cold storage of organs, maintaining physiological conditions during prese...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Copernicus Publications
2016-08-01
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| Series: | Journal of Sensors and Sensor Systems |
| Online Access: | http://www.j-sens-sens-syst.net/5/313/2016/jsss-5-313-2016.pdf |
| Summary: | The development of improved preservation techniques and the reliable
assessment of donor grafts are main fields of research in transplantation
medicine. Normothermic machine perfusion (NMP) is a promising alternative to
static cold storage of organs, maintaining physiological conditions during
preservation. In combination with NMP, we introduce hyperspectral imaging (HSI) as a novel approach for the monitoring of physiological kidney
parameters. A line-scan HSI camera system was used to record images of porcine
kidneys during NMP. Based on a dual-wavelength algorithm, the oxygen
saturation levels were calculated from HSI recordings. Furthermore, we
observed HSI images in the near-infrared (NIR) range in order to detect water
characteristics of the kidney tissue. We found increasing levels of
oxygenation during NMP and could discriminate between perfused and
non-perfused areas. Cysts at the renal capsula were characterized by an
absorption increase in the NIR band. Within this work, we showed that HSI is
able to detect relevant chemical changes during NMP and allows the
identification of pathologic variations. |
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| ISSN: | 2194-8771 2194-878X |