Detection of genes mediating beta-lactamase production in isolates of enterobacteria recovered from wild pets in Saudi Arabia

• Main Authors: Sabry A. Hassan, Mohamed Y. Shobrak
• Format: Article
• Language: English
• Published: Veterinary World 2015-12-01
• Series: Veterinary World
• Subjects: animal exhibit; extended-spectrum β-lactamases/AmpC beta-lactamase; fecal samples; polymerase chain reaction; Saudi Arabia
• Online Access: http://www.veterinaryworld.org/Vol.8/December-2015/6.pdf

Aim: To determine the genetic basis and types of beta-lactamase encountered among enterobacterial isolates of wild pets from the animal exhibit. Materials and Methods: A total of 17 beta-lactamase-producing enterobacteria recovered from fecal samples of wild pet animals were analyzed for a selected beta-lactamase gene by polymerase chain reaction. Results: Molecular analysis identified one or more β-lactamase-encoding genes in 14 enterobacterial isolates as a single or gene combination. The most frequent extended-spectrum β-lactamases types were transmission electron microscopy and CTX-M, and the most common AmpC enzymes were CMY-2 and DHA types. Conclusions: The study is the first in Saudi Arabia, have established the presence of β-lactamase-encoding genes in the fecal isolates of wild pets.