Jellyfish mucin may have potential disease-modifying effects on osteoarthritis

<p>Abstract</p> <p>Background</p> <p>We aimed to study the effects of intra-articular injection of jellyfish mucin (qniumucin) on articular cartilage degeneration in a model of osteoarthritis (OA) created in rabbit knees by resection of the anterior cruciate ligament. Q...

Full description

Bibliographic Details
Main Authors: Urai Makoto, Taniguchi Kayoko, Baba Takayuki, Kokubo Mami, Ushida Kiminori, Sato Masato, Ohta Naoshi, Kihira Koji, Mochida Joji
Format: Article
Language:English
Published: BMC 2009-12-01
Series:BMC Biotechnology
Online Access:http://www.biomedcentral.com/1472-6750/9/98
id doaj-0c9d077065f543cba994bf6ab602f837
record_format Article
spelling doaj-0c9d077065f543cba994bf6ab602f8372020-11-25T01:24:19ZengBMCBMC Biotechnology1472-67502009-12-01919810.1186/1472-6750-9-98Jellyfish mucin may have potential disease-modifying effects on osteoarthritisUrai MakotoTaniguchi KayokoBaba TakayukiKokubo MamiUshida KiminoriSato MasatoOhta NaoshiKihira KojiMochida Joji<p>Abstract</p> <p>Background</p> <p>We aimed to study the effects of intra-articular injection of jellyfish mucin (qniumucin) on articular cartilage degeneration in a model of osteoarthritis (OA) created in rabbit knees by resection of the anterior cruciate ligament. Qniumucin was extracted from <it>Aurelia aurita </it>(moon jellyfish) and <it>Stomolophus nomurai </it>(Nomura's jellyfish) and purified by ion exchange chromatography. The OA model used 36 knees in 18 Japanese white rabbits. Purified qniumucin extracts from <it>S. nomurai </it>or <it>A. aurita </it>were used at 1 mg/ml. Rabbits were divided into four groups: a control (C) group injected with saline; a hyaluronic acid (HA)-only group (H group); two qniumucin-only groups (M groups); and two qniumucin + HA groups (MH groups). One milligram of each solution was injected intra-articularly once a week for 5 consecutive weeks, starting from 4 weeks after surgery. Ten weeks after surgery, the articular cartilage was evaluated macroscopically and histologically.</p> <p>Results</p> <p>In the C and M groups, macroscopic cartilage defects extended to the subchondral bone medially and laterally. When the H and both MH groups were compared, only minor cartilage degeneration was observed in groups treated with qniumucin in contrast to the group without qniumucin. Histologically, densely safranin-O-stained cartilage layers were observed in the H and two MH groups, but cartilage was strongly maintained in both MH groups.</p> <p>Conclusion</p> <p>At the concentrations of qniumucin used in this study, injection together with HA inhibited articular cartilage degeneration in this model of OA.</p> http://www.biomedcentral.com/1472-6750/9/98
collection DOAJ
language English
format Article
sources DOAJ
author Urai Makoto
Taniguchi Kayoko
Baba Takayuki
Kokubo Mami
Ushida Kiminori
Sato Masato
Ohta Naoshi
Kihira Koji
Mochida Joji
spellingShingle Urai Makoto
Taniguchi Kayoko
Baba Takayuki
Kokubo Mami
Ushida Kiminori
Sato Masato
Ohta Naoshi
Kihira Koji
Mochida Joji
Jellyfish mucin may have potential disease-modifying effects on osteoarthritis
BMC Biotechnology
author_facet Urai Makoto
Taniguchi Kayoko
Baba Takayuki
Kokubo Mami
Ushida Kiminori
Sato Masato
Ohta Naoshi
Kihira Koji
Mochida Joji
author_sort Urai Makoto
title Jellyfish mucin may have potential disease-modifying effects on osteoarthritis
title_short Jellyfish mucin may have potential disease-modifying effects on osteoarthritis
title_full Jellyfish mucin may have potential disease-modifying effects on osteoarthritis
title_fullStr Jellyfish mucin may have potential disease-modifying effects on osteoarthritis
title_full_unstemmed Jellyfish mucin may have potential disease-modifying effects on osteoarthritis
title_sort jellyfish mucin may have potential disease-modifying effects on osteoarthritis
publisher BMC
series BMC Biotechnology
issn 1472-6750
publishDate 2009-12-01
description <p>Abstract</p> <p>Background</p> <p>We aimed to study the effects of intra-articular injection of jellyfish mucin (qniumucin) on articular cartilage degeneration in a model of osteoarthritis (OA) created in rabbit knees by resection of the anterior cruciate ligament. Qniumucin was extracted from <it>Aurelia aurita </it>(moon jellyfish) and <it>Stomolophus nomurai </it>(Nomura's jellyfish) and purified by ion exchange chromatography. The OA model used 36 knees in 18 Japanese white rabbits. Purified qniumucin extracts from <it>S. nomurai </it>or <it>A. aurita </it>were used at 1 mg/ml. Rabbits were divided into four groups: a control (C) group injected with saline; a hyaluronic acid (HA)-only group (H group); two qniumucin-only groups (M groups); and two qniumucin + HA groups (MH groups). One milligram of each solution was injected intra-articularly once a week for 5 consecutive weeks, starting from 4 weeks after surgery. Ten weeks after surgery, the articular cartilage was evaluated macroscopically and histologically.</p> <p>Results</p> <p>In the C and M groups, macroscopic cartilage defects extended to the subchondral bone medially and laterally. When the H and both MH groups were compared, only minor cartilage degeneration was observed in groups treated with qniumucin in contrast to the group without qniumucin. Histologically, densely safranin-O-stained cartilage layers were observed in the H and two MH groups, but cartilage was strongly maintained in both MH groups.</p> <p>Conclusion</p> <p>At the concentrations of qniumucin used in this study, injection together with HA inhibited articular cartilage degeneration in this model of OA.</p>
url http://www.biomedcentral.com/1472-6750/9/98
work_keys_str_mv AT uraimakoto jellyfishmucinmayhavepotentialdiseasemodifyingeffectsonosteoarthritis
AT taniguchikayoko jellyfishmucinmayhavepotentialdiseasemodifyingeffectsonosteoarthritis
AT babatakayuki jellyfishmucinmayhavepotentialdiseasemodifyingeffectsonosteoarthritis
AT kokubomami jellyfishmucinmayhavepotentialdiseasemodifyingeffectsonosteoarthritis
AT ushidakiminori jellyfishmucinmayhavepotentialdiseasemodifyingeffectsonosteoarthritis
AT satomasato jellyfishmucinmayhavepotentialdiseasemodifyingeffectsonosteoarthritis
AT ohtanaoshi jellyfishmucinmayhavepotentialdiseasemodifyingeffectsonosteoarthritis
AT kihirakoji jellyfishmucinmayhavepotentialdiseasemodifyingeffectsonosteoarthritis
AT mochidajoji jellyfishmucinmayhavepotentialdiseasemodifyingeffectsonosteoarthritis
_version_ 1725117887523323904