Jellyfish mucin may have potential disease-modifying effects on osteoarthritis
<p>Abstract</p> <p>Background</p> <p>We aimed to study the effects of intra-articular injection of jellyfish mucin (qniumucin) on articular cartilage degeneration in a model of osteoarthritis (OA) created in rabbit knees by resection of the anterior cruciate ligament. Q...
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doaj-0c9d077065f543cba994bf6ab602f8372020-11-25T01:24:19ZengBMCBMC Biotechnology1472-67502009-12-01919810.1186/1472-6750-9-98Jellyfish mucin may have potential disease-modifying effects on osteoarthritisUrai MakotoTaniguchi KayokoBaba TakayukiKokubo MamiUshida KiminoriSato MasatoOhta NaoshiKihira KojiMochida Joji<p>Abstract</p> <p>Background</p> <p>We aimed to study the effects of intra-articular injection of jellyfish mucin (qniumucin) on articular cartilage degeneration in a model of osteoarthritis (OA) created in rabbit knees by resection of the anterior cruciate ligament. Qniumucin was extracted from <it>Aurelia aurita </it>(moon jellyfish) and <it>Stomolophus nomurai </it>(Nomura's jellyfish) and purified by ion exchange chromatography. The OA model used 36 knees in 18 Japanese white rabbits. Purified qniumucin extracts from <it>S. nomurai </it>or <it>A. aurita </it>were used at 1 mg/ml. Rabbits were divided into four groups: a control (C) group injected with saline; a hyaluronic acid (HA)-only group (H group); two qniumucin-only groups (M groups); and two qniumucin + HA groups (MH groups). One milligram of each solution was injected intra-articularly once a week for 5 consecutive weeks, starting from 4 weeks after surgery. Ten weeks after surgery, the articular cartilage was evaluated macroscopically and histologically.</p> <p>Results</p> <p>In the C and M groups, macroscopic cartilage defects extended to the subchondral bone medially and laterally. When the H and both MH groups were compared, only minor cartilage degeneration was observed in groups treated with qniumucin in contrast to the group without qniumucin. Histologically, densely safranin-O-stained cartilage layers were observed in the H and two MH groups, but cartilage was strongly maintained in both MH groups.</p> <p>Conclusion</p> <p>At the concentrations of qniumucin used in this study, injection together with HA inhibited articular cartilage degeneration in this model of OA.</p> http://www.biomedcentral.com/1472-6750/9/98 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Urai Makoto Taniguchi Kayoko Baba Takayuki Kokubo Mami Ushida Kiminori Sato Masato Ohta Naoshi Kihira Koji Mochida Joji |
spellingShingle |
Urai Makoto Taniguchi Kayoko Baba Takayuki Kokubo Mami Ushida Kiminori Sato Masato Ohta Naoshi Kihira Koji Mochida Joji Jellyfish mucin may have potential disease-modifying effects on osteoarthritis BMC Biotechnology |
author_facet |
Urai Makoto Taniguchi Kayoko Baba Takayuki Kokubo Mami Ushida Kiminori Sato Masato Ohta Naoshi Kihira Koji Mochida Joji |
author_sort |
Urai Makoto |
title |
Jellyfish mucin may have potential disease-modifying effects on osteoarthritis |
title_short |
Jellyfish mucin may have potential disease-modifying effects on osteoarthritis |
title_full |
Jellyfish mucin may have potential disease-modifying effects on osteoarthritis |
title_fullStr |
Jellyfish mucin may have potential disease-modifying effects on osteoarthritis |
title_full_unstemmed |
Jellyfish mucin may have potential disease-modifying effects on osteoarthritis |
title_sort |
jellyfish mucin may have potential disease-modifying effects on osteoarthritis |
publisher |
BMC |
series |
BMC Biotechnology |
issn |
1472-6750 |
publishDate |
2009-12-01 |
description |
<p>Abstract</p> <p>Background</p> <p>We aimed to study the effects of intra-articular injection of jellyfish mucin (qniumucin) on articular cartilage degeneration in a model of osteoarthritis (OA) created in rabbit knees by resection of the anterior cruciate ligament. Qniumucin was extracted from <it>Aurelia aurita </it>(moon jellyfish) and <it>Stomolophus nomurai </it>(Nomura's jellyfish) and purified by ion exchange chromatography. The OA model used 36 knees in 18 Japanese white rabbits. Purified qniumucin extracts from <it>S. nomurai </it>or <it>A. aurita </it>were used at 1 mg/ml. Rabbits were divided into four groups: a control (C) group injected with saline; a hyaluronic acid (HA)-only group (H group); two qniumucin-only groups (M groups); and two qniumucin + HA groups (MH groups). One milligram of each solution was injected intra-articularly once a week for 5 consecutive weeks, starting from 4 weeks after surgery. Ten weeks after surgery, the articular cartilage was evaluated macroscopically and histologically.</p> <p>Results</p> <p>In the C and M groups, macroscopic cartilage defects extended to the subchondral bone medially and laterally. When the H and both MH groups were compared, only minor cartilage degeneration was observed in groups treated with qniumucin in contrast to the group without qniumucin. Histologically, densely safranin-O-stained cartilage layers were observed in the H and two MH groups, but cartilage was strongly maintained in both MH groups.</p> <p>Conclusion</p> <p>At the concentrations of qniumucin used in this study, injection together with HA inhibited articular cartilage degeneration in this model of OA.</p> |
url |
http://www.biomedcentral.com/1472-6750/9/98 |
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