| Summary: | Thirty-six pregnant Holstein⁻Friesian cows were used to study the effect of Yerba Mate (YM) supplementation during the dry period on redox balance. The treatments groups were Control (no YM), YM 250 (250 g/cow/day), and YM 500 (500 g/cow/day). Blood samples were obtained 30 days prepartum, at calving, and monthly postpartum until four months post calving. Liveweight (LW) and body condition score (BCS) were assessed prepartum, at calving, and then postpartum monthly until the end of the trial. Plasma was analyzed for hydroperoxides (d-ROMs), advanced oxidation protein products (AOPP), and biological antioxidant potential (BAP). The oxidative stress index (OSI) was calculated as OSI = ROMs/BAP × 100. Cows were milked twice daily, and milk yield data were recorded daily. Redox balance was improved by YM supplementation, as reflected in the lower OSI values observed in the YM groups. Yerba Mate supplementation significantly affected LW, but did not affect BCS. Milk yield averaged 28.1 ± 0.40, 29.0 ± 0.48, and 29.9 ± 0.46 L/cow/day in the Control, YM 250, and YM 500 groups, respectively, but was not significant. Nutritional manipulation during the dry period with Yerba Mate has demonstrated the potential to improve redox balance and milk yield.
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