Study on the polymorphism of ITS in Osmanthus

• Main Authors: Lu Zhiyuan, Gao Wujun, Li Shufen, Sun Xiao
• Format: Article
• Language: English
• Published: "Alexandru Ioan Cuza" University of Iași 2009-03-01
• Series: Analele Ştiinţifice Ale Universităţii Alexandru Ioan Cuza din Iași,Sectiunea II A : Genetica si Biologie Moleculara
• Online Access: http://www.gbm.bio.uaic.ro/index.php/gbm/article/view/441

In this study, ITS sequence was cloned respectively from 17 species of Osmanthus and sequenced. It was found that length of ITS sequence including 5.8S of 17 species ranged from 614bp to 619bp, 5.8S was composed of 163bp. Furthermore, ITS sequence of individuals species took on polymorphism, the similarity coefficient between ITS sequences of every species was 97.90%~100%, the minimum similarity coefficient appeared in O.reticulatus (97.90%~99.50%), and the maximum is shown in O.cooperi (99.70%~100%). 58 kinds of ITS sequences from 17 species were analyzed and discovered that the polymorphic ITS sequences from 12 species were respectively clustered together, but 3 kinds of ITS sequences from O.Venosus were not clustered together, thus apart from O.Venosus, any of ITS sequence clustered together from the other 16 species of Osmanthus all could stand for the feature of ITS sequence in every species. In addition, in the dendrogram, O.yunnanensis and O.attenuatus were clustered to be one group, O.americanus, O.matsumuraanus and O.delavayi were clustered to different groups, the other 12 species of were clustered together, which is partly identical with the viewpoint of traditional taxology about Osmanthus. Therefore, it might be feasible to analyze the sibship and phylogenetic relationships of Osmanthus by ITS.