Prophylactic effect of Kudingcha polyphenols on oxazolone induced colitis through its antioxidant capacities

• Main Authors: Xingyao Long, Yanni Pan, Xin Zhao
• Format: Article
• Language: English
• Published: KeAi Communications Co., Ltd. 2018-09-01
• Series: Food Science and Human Wellness
• Online Access: http://www.sciencedirect.com/science/article/pii/S2213453017302045

This research the preventive effect of Kudingcha polyphenols (Kp) on colitis based on animal experiments. Experimental mice divided into four groups, including normal group, model group, low-concentration Kp (LKP) group and high-concentration Kp (HKP) group, and they all smeared and given 0.15 mL 1% oxazolone solution by lavage to induce BALB/c mice colitis. DAI, colon weight/length ratio, serum levels of cytokines, related antioxidant activities of colon tissues such, and the mRNA expressions. The experimental results show that KP can significantly (p < 0.05) reduce DAI in mice with colitis and inhibit shortening of colon caused by colitis, increase colon weight/length ratio. It can also significantly reduce (p < 0.05) the activities of MPO, NO, MDA in mice colon tissues with colitis, increase the activity of GSH, and significantly increase (p < 0.05) the level of IL-2 and decrease the level of IL-10 in mice serum with colitis. The results of qPCR assay show that Kp can significantly increase (p < 0.05) the expression of Cu/Zn-SOD, Mn-SOD, GSH-Px, CAT, c-Kit, SCF and decrease the expression of IL-8, CXCR2 in mice colon tissues with colitis. Kp has a good preventive effect on colitis, and high concentration of PT has better preventive effect. Keywords: Kudingcha polyphenols, Oxazolone, Antioxidant, Colitis, mRNA expression