cDNA and deduced primary structure of basic phospholipase A2 with neurotoxic activity from the venom secretion of the Crotalus durissus collilineatus rattlesnake

cDNA and deduced primary structure of basic phospholipase A2 with neurotoxic activity from the venom secretion of the Crotalus durissus collilineatus rattlesnake

To illustrate the construction of precursor complementary DNAs, we isolated mRNAs from whole venom samples. After reverse transcription polymerase chain reaction (RT-PCR), we amplified the cDNA coding for a neurotoxic protein, phospholipase A2 D49 (PLA2 D49), from the venom of Crotalus durissus coll...

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Bibliographic Details
Main Authors: F.H.R. Fagundes, M. Oliveira, S. Huancahuire-Vega, F.F. Romero-Vargas, L.A. Ponce-Soto, S. Marangoni
Format: Article
Language:English
Published: Associação Brasileira de Divulgação Científica 2010-03-01
Series:Brazilian Journal of Medical and Biological Research
Subjects:
Cdc PLA2
cDNA
Chick biventer cervicis
Crotalus durissus collilineatus
Mass spectrometry MALDI-TOF
Neurotoxic agents
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000300007

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