High Performance Liquid Chromatography in Quantitative Assessment of Diosaginine Metabolic Output Isolated from Callus Cultures, Cellular Pendants and Root Poems in Medical Plant Ring

• Main Authors: Mzahim Al Malla, Luma Al-Zonbaidi
• Format: Article
• Language: Arabic
• Published: College of Education for Pure Sciences 2007-01-01
• Series: مجلة التربية والعلم
• Subjects: chromatography; diosaginine; callus cultures
• Online Access: https://edusj.mosuljournals.com/article_51018_9724a4527a2974613a2b05d6012942f8.pdf

ABSTRACT<br /> Callus cultures were obtained from stem and leaf segments of Trigonella foenum-graecum grown on Murashige and Skoog (MS) medium supplemented with combinations of Benzyl adenine (BA), Naphthaleneacetic acid (NAA). Maximum callusing 97% were obtained from stem segments grown on MS medium supplemented with a mixture of NAA 1.0 mg L-1 and BA 2.0 mg L-1. The friable callus was reasonable for cell suspension cultures. Liquid MS and B5 media with addition of NAA, BA, 2,4-D and Kinetin were used for induction of these cultures. Maximum and steadily growth of these suspensions were found on liquid B5 medium supplemented with 2,4-D (0.2 mg L-1) and Kin. (1.5 mg L-1 ) with leaf callus. The study established also a culture of hairy roots developed from those adventitious roots induced on intact fenugreek seedlings inoculated by Agrobacterium rhizogenes R1601. <br /> High performance liquid chromatography analysis confirmed the presence of diosgenin in callus at ratio 46.3%, In liquid medium of cell suspension derived from leaf callus at ratio of 50.2%, that of stem callus 24.1% . The ratio was at 21.79% of hairy root culture.