Effect of Terpenoids and Flavonoids Isolated from <i>Baccharis conferta</i> Kunth on TPA-Induced Ear Edema in Mice

• Main Authors: Gutiérrez-Román Ana Silvia, Trejo-Tapia Gabriela, Herrera-Ruiz Maribel, Monterrosas-Brisson Nayeli, Trejo-Espino José Luis, Zamilpa Alejandro, González-Cortazar Manasés
• Format: Article
• Language: English
• Published: MDPI AG 2020-03-01
• Series: Molecules
• Subjects: diterpenes; flavonoids; topical anti-inflammatory; bacchofertone; <i>baccharis conferta</i> kunth
• Online Access: https://www.mdpi.com/1420-3049/25/6/1379

In this study, we isolated from the aerial parts of <i>Baccharis conferta</i> Kunth (i) a new neoclerodane, denominated &#8220;bacchofertone&#8221;; (ii) four known terpenes: schensianol A, bacchofertin, kingidiol and oleanolic acid; and (iii) two flavonoids: cirsimaritin and hispidulin. All structures were identified by an exhaustive analysis of nuclear magnetic resonance (NMR) and mass spectroscopy (MS). Extracts from aerial parts were screened for anti-inflammatory activity in the mice ear edema model of 12-<i>O</i>-tetradecanoylforbol-13-acetate mice. Dichloromethane extract (BcD) exhibited 78.5 &#177; 0.72% inhibition of edema, followed by the BcD2 and BcD3 fractions of 71.4% and 82.9% respectively, at a dose of 1 mg/ear. Kingidiol and cirsimaritin were the most potent compounds identified, with a median effective dose of 0.12 and 0.16 mg/ear, respectively. A histological analysis showed that the topical application of TPA promoted intense cell infiltration, and this inflammatory parameter was reduced with the topical application of isolated compounds.