Antibacterial Activity of TiO<sub>2</sub> Nanoparticles Prepared by One-Step Laser Ablation in Liquid

Laser ablation in liquid was utilized to prepare a TiO<sub>2</sub> NP suspension in in deionized distilled water using Q-switch Nd: YAG laser at various laser energies and ablation times. The samples were characterized using UV–visible absorption spectra obtained with a UV–visible spectr...

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Main Authors:	Khawla S. Khashan, Ghassan M. Sulaiman, Farah A. Abdulameer, Salim Albukhaty, Mohammed A. Ibrahem, Tahani Al-Muhimeed, Abeer A. AlObaid
Format:	Article
Language:	English
Published:	MDPI AG 2021-05-01
Series:	Applied Sciences
Subjects:	TiO<sub>2</sub> NPs laser ablation in liquid characterization antibacterial activity
Online Access:	https://www.mdpi.com/2076-3417/11/10/4623

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spelling	doaj-0beb03f161b64f418ad0119b75fed12b2021-06-01T00:25:54ZengMDPI AGApplied Sciences2076-34172021-05-01114623462310.3390/app11104623Antibacterial Activity of TiO<sub>2</sub> Nanoparticles Prepared by One-Step Laser Ablation in LiquidKhawla S. Khashan0Ghassan M. Sulaiman1Farah A. Abdulameer2Salim Albukhaty3Mohammed A. Ibrahem4Tahani Al-Muhimeed5Abeer A. AlObaid6Division of Laser Sciences and Technology, Department of Applied Sciences, University of Technology, Baghdad 10066, IraqDivision of Biotechnology, Department of Applied Sciences, University of Technology, Baghdad 10066, IraqCollege of Health and Medical Techniques, Gilgamesh Ahliya University, Baghdad 10022, IraqDepartment of Chemistry, College of Science, University of Misan, Maysan, Amarah 62001, IraqDivision of Laser Sciences and Technology, Department of Applied Sciences, University of Technology, Baghdad 10066, IraqDepartment of Chemistry, College of Science, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi ArabiaDepartment of Chemistry, College of Science, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi ArabiaLaser ablation in liquid was utilized to prepare a TiO<sub>2</sub> NP suspension in in deionized distilled water using Q-switch Nd: YAG laser at various laser energies and ablation times. The samples were characterized using UV–visible absorption spectra obtained with a UV–visible spectrophotometer (UV-Vis,) Fourier transform infrared (FTIR), X-ray diffraction (XRD), and transmission electron microscope (TEM). While, UV-Vis spectra showed the characteristic band-to-band absorption peak of TiO<sub>2</sub> NPs in the UV range. FTIR analysis showed the existence of O-Ti-O bond. XRD patterns indicated the presence of (101) and (112) plane crystalline phases of TiO<sub>2</sub>. TEM images showed a spherical-like structure of TiO<sub>2</sub> NPs with various size distributions depending on the ablation period. It was also found that there is a relationship between laser ablation time and TiO<sub>2</sub> NP size distribution, where longer ablation times led to the smaller size distribution. The antibacterial activity of TiO<sub>2</sub> NPs was evaluated with different species of bacteria such as <i>Escherichia coli</i>, <i>Pseudomonas aeruginosa</i>, <i>Proteus vulgaris</i>, and <i>Staphylococcus aureus</i>, using the liquid approach. The optimum activity of TiO<sub>2</sub> NPs is found to be against <i>E. coli</i> at 1000 μg mL<sup>−1</sup>. Furthermore, adding, TiO<sub>2</sub> NPs (1000 μg mL<sup>−1</sup>) in the presence of amoxicillin has a synergic effect on <i>E. coli</i> and <i>S. aureus</i> growth, as measured by the well diffusion method. However, both <i>E. coli</i> (11.6 ± 0.57mm) and <i>S. aureus</i> (13.3 ± 0.57mm) were inhibited by this process.https://www.mdpi.com/2076-3417/11/10/4623TiO<sub>2</sub> NPslaser ablation in liquidcharacterizationantibacterial activity
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Khawla S. Khashan Ghassan M. Sulaiman Farah A. Abdulameer Salim Albukhaty Mohammed A. Ibrahem Tahani Al-Muhimeed Abeer A. AlObaid
spellingShingle	Khawla S. Khashan Ghassan M. Sulaiman Farah A. Abdulameer Salim Albukhaty Mohammed A. Ibrahem Tahani Al-Muhimeed Abeer A. AlObaid Antibacterial Activity of TiO<sub>2</sub> Nanoparticles Prepared by One-Step Laser Ablation in Liquid Applied Sciences TiO<sub>2</sub> NPs laser ablation in liquid characterization antibacterial activity
author_facet	Khawla S. Khashan Ghassan M. Sulaiman Farah A. Abdulameer Salim Albukhaty Mohammed A. Ibrahem Tahani Al-Muhimeed Abeer A. AlObaid
author_sort	Khawla S. Khashan
title	Antibacterial Activity of TiO<sub>2</sub> Nanoparticles Prepared by One-Step Laser Ablation in Liquid
title_short	Antibacterial Activity of TiO<sub>2</sub> Nanoparticles Prepared by One-Step Laser Ablation in Liquid
title_full	Antibacterial Activity of TiO<sub>2</sub> Nanoparticles Prepared by One-Step Laser Ablation in Liquid
title_fullStr	Antibacterial Activity of TiO<sub>2</sub> Nanoparticles Prepared by One-Step Laser Ablation in Liquid
title_full_unstemmed	Antibacterial Activity of TiO<sub>2</sub> Nanoparticles Prepared by One-Step Laser Ablation in Liquid
title_sort	antibacterial activity of tio<sub>2</sub> nanoparticles prepared by one-step laser ablation in liquid
publisher	MDPI AG
series	Applied Sciences
issn	2076-3417
publishDate	2021-05-01
description	Laser ablation in liquid was utilized to prepare a TiO<sub>2</sub> NP suspension in in deionized distilled water using Q-switch Nd: YAG laser at various laser energies and ablation times. The samples were characterized using UV–visible absorption spectra obtained with a UV–visible spectrophotometer (UV-Vis,) Fourier transform infrared (FTIR), X-ray diffraction (XRD), and transmission electron microscope (TEM). While, UV-Vis spectra showed the characteristic band-to-band absorption peak of TiO<sub>2</sub> NPs in the UV range. FTIR analysis showed the existence of O-Ti-O bond. XRD patterns indicated the presence of (101) and (112) plane crystalline phases of TiO<sub>2</sub>. TEM images showed a spherical-like structure of TiO<sub>2</sub> NPs with various size distributions depending on the ablation period. It was also found that there is a relationship between laser ablation time and TiO<sub>2</sub> NP size distribution, where longer ablation times led to the smaller size distribution. The antibacterial activity of TiO<sub>2</sub> NPs was evaluated with different species of bacteria such as <i>Escherichia coli</i>, <i>Pseudomonas aeruginosa</i>, <i>Proteus vulgaris</i>, and <i>Staphylococcus aureus</i>, using the liquid approach. The optimum activity of TiO<sub>2</sub> NPs is found to be against <i>E. coli</i> at 1000 μg mL<sup>−1</sup>. Furthermore, adding, TiO<sub>2</sub> NPs (1000 μg mL<sup>−1</sup>) in the presence of amoxicillin has a synergic effect on <i>E. coli</i> and <i>S. aureus</i> growth, as measured by the well diffusion method. However, both <i>E. coli</i> (11.6 ± 0.57mm) and <i>S. aureus</i> (13.3 ± 0.57mm) were inhibited by this process.
topic	TiO<sub>2</sub> NPs laser ablation in liquid characterization antibacterial activity
url	https://www.mdpi.com/2076-3417/11/10/4623
work_keys_str_mv	AT khawlaskhashan antibacterialactivityoftiosub2subnanoparticlespreparedbyonesteplaserablationinliquid AT ghassanmsulaiman antibacterialactivityoftiosub2subnanoparticlespreparedbyonesteplaserablationinliquid AT farahaabdulameer antibacterialactivityoftiosub2subnanoparticlespreparedbyonesteplaserablationinliquid AT salimalbukhaty antibacterialactivityoftiosub2subnanoparticlespreparedbyonesteplaserablationinliquid AT mohammedaibrahem antibacterialactivityoftiosub2subnanoparticlespreparedbyonesteplaserablationinliquid AT tahanialmuhimeed antibacterialactivityoftiosub2subnanoparticlespreparedbyonesteplaserablationinliquid AT abeeraalobaid antibacterialactivityoftiosub2subnanoparticlespreparedbyonesteplaserablationinliquid
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Antibacterial Activity of TiO<sub>2</sub> Nanoparticles Prepared by One-Step Laser Ablation in Liquid

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