Optimum range of plasmid supercoiled DNA for preparation of ccompetent Top 10 E. coli

Objectives: In-house preparation of chemically competent andelectrocompetent Top 10 E. coli is not only economical butmeets the needs for most of the molecular cloning work. Forsuch transformations an optimum range of plasmidsupercoiled DNA is needed. Therefore, the present studydescribes the modifi...

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Bibliographic Details
Main Authors: Muhammad Tahir Majeed, Khalid Hussain, Mamoona Noreen, Mateen Izhar
Format: Article
Language:English
Published: Archives of Global Professionals 2011-05-01
Series:Archives of Pharmacy Practice
Subjects:
Online Access:http://www.archivepp.com/Resources/Files/652011151834731.pdf