A Comparison of Molecular Typing Methods Applied to Enterobacter cloacae complex: hsp60 Sequencing, Rep-PCR, and MLST

A Comparison of Molecular Typing Methods Applied to Enterobacter cloacae complex: hsp60 Sequencing, Rep-PCR, and MLST

Molecular typing using repetitive sequenced-based PCR (rep-PCR) and hsp60 sequencing were applied to a collection of diverse Enterobacter cloacae complex isolates. To determine the most practical method for reference laboratories, we analyzed 71 E. cloacae complex isolates from sporadic and outbreak...

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Main Authors: Roberto Viau, Lee M. Kiedrowski, Barry N. Kreiswirth, Mark Adams, Federico Perez, Dror Marchaim, Dubert M. Guerrero, Keith S. Kaye, Latania K. Logan, Maria Virginia Villegas, Robert A. Bonomo
Format: Article
Language:English
Published: Case Western Reserve University 2017-02-01
Series:Pathogens and Immunity
Subjects:
Enterobacter cloacae
Bacterial Typing Technique
Molecular Epidemiology
Molecular typing
DNA fingerprinting
Online Access:https://paijournal.com/index.php/paijournal/article/view/99
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spelling doaj-0bddb5e9ec9a47c79956baadb0a9f2fa2020-11-24T21:49:58ZengCase Western Reserve UniversityPathogens and Immunity2469-29642017-02-0121233310.20411/pai.v1i2.9942A Comparison of Molecular Typing Methods Applied to Enterobacter cloacae complex: hsp60 Sequencing, Rep-PCR, and MLSTRoberto Viau0Lee M. Kiedrowski1Barry N. Kreiswirth2Mark Adams3Federico Perez4Dror Marchaim5Dubert M. Guerrero6Keith S. Kaye7Latania K. Logan8Maria Virginia Villegas9Robert A. Bonomo10Louis Stokes Cleveland Dept. Of Veterans Affairs Medical Center, Department of Medicine. Case Western Reserve University, Cleveland, OHNorth Dakota State University, Fargo, NDPublic Health Research Institute Tuberculosis Center, Rutgers University, Newark, NJJ. Craig Venter Institute, La Jolla, CAMedical and Research Services, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, OHDepartment of Medicine, Infection Control and Prevention Unit of Infectious Diseases, Assaf Harofeh Medical Center, Israel.Sanford Health, Fargo, Department of Infectious Disease, Fargo, NDWayne State University and Detroit Medical Center, Detroit, MIMedical and Research Services Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, OH; Rush University Medical Center, Chicago, ILCentro Internacional de Entrenamiento e Investigaciones Médicas (CIDEIM), Cali, Colombiai, and Departments of Medicine, Microbiology and Cellular Biology, and Pharmacology, Case Western Reserve University School of Medicine, Cleveland, OHMedical and Research Services Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, OH; Departments of Medicine, Microbiology and Cellular Biology, and Pharmacology, Case Western Reserve University School of Medicine, Cleveland, OHMolecular typing using repetitive sequenced-based PCR (rep-PCR) and hsp60 sequencing were applied to a collection of diverse Enterobacter cloacae complex isolates. To determine the most practical method for reference laboratories, we analyzed 71 E. cloacae complex isolates from sporadic and outbreak occurrences originating from 4 geographic areas. While rep-PCR was more discriminating, hsp60 sequencing provided a broader and a more objective geographical tracking method similar to multilocus sequence typing (MLST). In addition, we suggest that MLST may have higher discriminative power compared to hsp60 sequencing, although rep-PCR remains the most discriminative method for local outbreak investigations. In addition, rep-PCR can be an effective and inexpensive method for local outbreak investigation.https://paijournal.com/index.php/paijournal/article/view/99Enterobacter cloacaeBacterial Typing TechniqueMolecular EpidemiologyMolecular typingDNA fingerprinting
collection DOAJ
language English
format Article
sources DOAJ
author Roberto Viau
Lee M. Kiedrowski
Barry N. Kreiswirth
Mark Adams
Federico Perez
Dror Marchaim
Dubert M. Guerrero
Keith S. Kaye
Latania K. Logan
Maria Virginia Villegas
Robert A. Bonomo
spellingShingle Roberto Viau
Lee M. Kiedrowski
Barry N. Kreiswirth
Mark Adams
Federico Perez
Dror Marchaim
Dubert M. Guerrero
Keith S. Kaye
Latania K. Logan
Maria Virginia Villegas
Robert A. Bonomo
A Comparison of Molecular Typing Methods Applied to Enterobacter cloacae complex: hsp60 Sequencing, Rep-PCR, and MLST
Pathogens and Immunity
Enterobacter cloacae
Bacterial Typing Technique
Molecular Epidemiology
Molecular typing
DNA fingerprinting
author_facet Roberto Viau
Lee M. Kiedrowski
Barry N. Kreiswirth
Mark Adams
Federico Perez
Dror Marchaim
Dubert M. Guerrero
Keith S. Kaye
Latania K. Logan
Maria Virginia Villegas
Robert A. Bonomo
author_sort Roberto Viau
title A Comparison of Molecular Typing Methods Applied to Enterobacter cloacae complex: hsp60 Sequencing, Rep-PCR, and MLST
title_short A Comparison of Molecular Typing Methods Applied to Enterobacter cloacae complex: hsp60 Sequencing, Rep-PCR, and MLST
title_full A Comparison of Molecular Typing Methods Applied to Enterobacter cloacae complex: hsp60 Sequencing, Rep-PCR, and MLST
title_fullStr A Comparison of Molecular Typing Methods Applied to Enterobacter cloacae complex: hsp60 Sequencing, Rep-PCR, and MLST
title_full_unstemmed A Comparison of Molecular Typing Methods Applied to Enterobacter cloacae complex: hsp60 Sequencing, Rep-PCR, and MLST
title_sort comparison of molecular typing methods applied to enterobacter cloacae complex: hsp60 sequencing, rep-pcr, and mlst
publisher Case Western Reserve University
series Pathogens and Immunity
issn 2469-2964
publishDate 2017-02-01
description Molecular typing using repetitive sequenced-based PCR (rep-PCR) and hsp60 sequencing were applied to a collection of diverse Enterobacter cloacae complex isolates. To determine the most practical method for reference laboratories, we analyzed 71 E. cloacae complex isolates from sporadic and outbreak occurrences originating from 4 geographic areas. While rep-PCR was more discriminating, hsp60 sequencing provided a broader and a more objective geographical tracking method similar to multilocus sequence typing (MLST). In addition, we suggest that MLST may have higher discriminative power compared to hsp60 sequencing, although rep-PCR remains the most discriminative method for local outbreak investigations. In addition, rep-PCR can be an effective and inexpensive method for local outbreak investigation.
topic Enterobacter cloacae
Bacterial Typing Technique
Molecular Epidemiology
Molecular typing
DNA fingerprinting
url https://paijournal.com/index.php/paijournal/article/view/99
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