Effects of <i>Bacillus subtilis</i> WB60 and <i>Lactococcus lactis</i> on Growth, Immune Responses, Histology and Gene Expression in Nile Tilapia, <i>Oreochromis niloticus</i>

An eight-week feeding trial was conducted to evaluate the effects of a basal control diet (CON), <i>Bacillus subtilis</i> at 10<sup>7</sup> (BS<sub>7</sub>) and at 10<sup>8</sup> CFU/g diet (BS<sub>8</sub>), <i>Lactococcus lactis</...

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Main Authors: Seonghun Won, Ali Hamidoghli, Wonsuk Choi, Youngjin Park, Won Je Jang, In-Soo Kong, Sungchul C. Bai
Format: Article
Language:English
Published: MDPI AG 2020-01-01
Series:Microorganisms
Subjects:
Online Access:https://www.mdpi.com/2076-2607/8/1/67
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spelling doaj-0bcf65c8436e4a79ac1f3c55261ad5712020-11-25T02:23:44ZengMDPI AGMicroorganisms2076-26072020-01-01816710.3390/microorganisms8010067microorganisms8010067Effects of <i>Bacillus subtilis</i> WB60 and <i>Lactococcus lactis</i> on Growth, Immune Responses, Histology and Gene Expression in Nile Tilapia, <i>Oreochromis niloticus</i>Seonghun Won0Ali Hamidoghli1Wonsuk Choi2Youngjin Park3Won Je Jang4In-Soo Kong5Sungchul C. Bai6Department of Marine Bio-materials and Aquaculture, Feeds and Foods Nutrition Research Center (FFNRC), Pukyong National University, Busan 608-737, KoreaDepartment of Marine Bio-materials and Aquaculture, Feeds and Foods Nutrition Research Center (FFNRC), Pukyong National University, Busan 608-737, KoreaDepartment of Marine Bio-materials and Aquaculture, Feeds and Foods Nutrition Research Center (FFNRC), Pukyong National University, Busan 608-737, KoreaFaculty of Biosciences and Aquaculture, Nord University, Universitetsallen 11, 8049 Bodo, NorwayDepartment of Biotechnology, Pukyong National University, Busan 608-737, KoreaDepartment of Biotechnology, Pukyong National University, Busan 608-737, KoreaDepartment of Marine Bio-materials and Aquaculture, Feeds and Foods Nutrition Research Center (FFNRC), Pukyong National University, Busan 608-737, KoreaAn eight-week feeding trial was conducted to evaluate the effects of a basal control diet (CON), <i>Bacillus subtilis</i> at 10<sup>7</sup> (BS<sub>7</sub>) and at 10<sup>8</sup> CFU/g diet (BS<sub>8</sub>), <i>Lactococcus lactis</i> at 10<sup>7</sup> CFU/g (LL<sub>7</sub>) and at 10<sup>8</sup> CFU/g diet (LL<sub>8</sub>), and oxytetracycline (OTC) at 4 g/kg diet on Nile tilapia. Fish with initial body weight of 2.83 &#177; 0.05 g (mean &#177; SD) were fed two times a day. Weight gain, specific growth rate, feed efficiency, protein efficiency ratio and lysozyme activity of fish fed BS<sub>8</sub>, LL<sub>8</sub> and LL<sub>7</sub> diets were significantly higher than those of fish fed CON diet (<i>p</i> &lt; 0.05). Superoxide dismutase and myeloperoxidase activity of fish fed BS<sub>8</sub>, LL<sub>8,</sub> BS<sub>7</sub>, LL<sub>7</sub> and OTC diets were significantly higher than those of fish fed CON diet. Intestinal villi length and muscular layer thickness of fish fed BS<sub>8</sub>, LL<sub>8</sub> and LL<sub>7</sub> diets were significantly higher than those of fish fed CON and OTC diets. Also, heat shock protein 70 (HSP70), interleukin (IL-1&#946;), interferon-gamma (IFN-&#947;) and tumour necrosis factor (TNF-&#945;) gene expression of fish fed BS<sub>8</sub> and LL<sub>8</sub> diets were significantly higher than those of fish fed CON diet. After 13 days of challenge test, cumulative survival rate of fish fed BS<sub>8</sub> and LL<sub>8</sub> diets were significantly higher than those of fish fed CON, BS<sub>7</sub> and OTC diets. Based on these results, <i>B. subtilis</i> and <i>L. lactis</i> at 10<sup>8</sup> (CFU/g) could replace antibiotics, and have beneficial effects on growth, immunity, histology, gene expression, and disease resistance in Nile tilapia.https://www.mdpi.com/2076-2607/8/1/67probiotic<i>bacillus</i><i>lactococcus</i>nile tilapiagrowthimmunity
collection DOAJ
language English
format Article
sources DOAJ
author Seonghun Won
Ali Hamidoghli
Wonsuk Choi
Youngjin Park
Won Je Jang
In-Soo Kong
Sungchul C. Bai
spellingShingle Seonghun Won
Ali Hamidoghli
Wonsuk Choi
Youngjin Park
Won Je Jang
In-Soo Kong
Sungchul C. Bai
Effects of <i>Bacillus subtilis</i> WB60 and <i>Lactococcus lactis</i> on Growth, Immune Responses, Histology and Gene Expression in Nile Tilapia, <i>Oreochromis niloticus</i>
Microorganisms
probiotic
<i>bacillus</i>
<i>lactococcus</i>
nile tilapia
growth
immunity
author_facet Seonghun Won
Ali Hamidoghli
Wonsuk Choi
Youngjin Park
Won Je Jang
In-Soo Kong
Sungchul C. Bai
author_sort Seonghun Won
title Effects of <i>Bacillus subtilis</i> WB60 and <i>Lactococcus lactis</i> on Growth, Immune Responses, Histology and Gene Expression in Nile Tilapia, <i>Oreochromis niloticus</i>
title_short Effects of <i>Bacillus subtilis</i> WB60 and <i>Lactococcus lactis</i> on Growth, Immune Responses, Histology and Gene Expression in Nile Tilapia, <i>Oreochromis niloticus</i>
title_full Effects of <i>Bacillus subtilis</i> WB60 and <i>Lactococcus lactis</i> on Growth, Immune Responses, Histology and Gene Expression in Nile Tilapia, <i>Oreochromis niloticus</i>
title_fullStr Effects of <i>Bacillus subtilis</i> WB60 and <i>Lactococcus lactis</i> on Growth, Immune Responses, Histology and Gene Expression in Nile Tilapia, <i>Oreochromis niloticus</i>
title_full_unstemmed Effects of <i>Bacillus subtilis</i> WB60 and <i>Lactococcus lactis</i> on Growth, Immune Responses, Histology and Gene Expression in Nile Tilapia, <i>Oreochromis niloticus</i>
title_sort effects of <i>bacillus subtilis</i> wb60 and <i>lactococcus lactis</i> on growth, immune responses, histology and gene expression in nile tilapia, <i>oreochromis niloticus</i>
publisher MDPI AG
series Microorganisms
issn 2076-2607
publishDate 2020-01-01
description An eight-week feeding trial was conducted to evaluate the effects of a basal control diet (CON), <i>Bacillus subtilis</i> at 10<sup>7</sup> (BS<sub>7</sub>) and at 10<sup>8</sup> CFU/g diet (BS<sub>8</sub>), <i>Lactococcus lactis</i> at 10<sup>7</sup> CFU/g (LL<sub>7</sub>) and at 10<sup>8</sup> CFU/g diet (LL<sub>8</sub>), and oxytetracycline (OTC) at 4 g/kg diet on Nile tilapia. Fish with initial body weight of 2.83 &#177; 0.05 g (mean &#177; SD) were fed two times a day. Weight gain, specific growth rate, feed efficiency, protein efficiency ratio and lysozyme activity of fish fed BS<sub>8</sub>, LL<sub>8</sub> and LL<sub>7</sub> diets were significantly higher than those of fish fed CON diet (<i>p</i> &lt; 0.05). Superoxide dismutase and myeloperoxidase activity of fish fed BS<sub>8</sub>, LL<sub>8,</sub> BS<sub>7</sub>, LL<sub>7</sub> and OTC diets were significantly higher than those of fish fed CON diet. Intestinal villi length and muscular layer thickness of fish fed BS<sub>8</sub>, LL<sub>8</sub> and LL<sub>7</sub> diets were significantly higher than those of fish fed CON and OTC diets. Also, heat shock protein 70 (HSP70), interleukin (IL-1&#946;), interferon-gamma (IFN-&#947;) and tumour necrosis factor (TNF-&#945;) gene expression of fish fed BS<sub>8</sub> and LL<sub>8</sub> diets were significantly higher than those of fish fed CON diet. After 13 days of challenge test, cumulative survival rate of fish fed BS<sub>8</sub> and LL<sub>8</sub> diets were significantly higher than those of fish fed CON, BS<sub>7</sub> and OTC diets. Based on these results, <i>B. subtilis</i> and <i>L. lactis</i> at 10<sup>8</sup> (CFU/g) could replace antibiotics, and have beneficial effects on growth, immunity, histology, gene expression, and disease resistance in Nile tilapia.
topic probiotic
<i>bacillus</i>
<i>lactococcus</i>
nile tilapia
growth
immunity
url https://www.mdpi.com/2076-2607/8/1/67
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