Effects of <i>Bacillus subtilis</i> WB60 and <i>Lactococcus lactis</i> on Growth, Immune Responses, Histology and Gene Expression in Nile Tilapia, <i>Oreochromis niloticus</i>
An eight-week feeding trial was conducted to evaluate the effects of a basal control diet (CON), <i>Bacillus subtilis</i> at 10<sup>7</sup> (BS<sub>7</sub>) and at 10<sup>8</sup> CFU/g diet (BS<sub>8</sub>), <i>Lactococcus lactis</...
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doaj-0bcf65c8436e4a79ac1f3c55261ad5712020-11-25T02:23:44ZengMDPI AGMicroorganisms2076-26072020-01-01816710.3390/microorganisms8010067microorganisms8010067Effects of <i>Bacillus subtilis</i> WB60 and <i>Lactococcus lactis</i> on Growth, Immune Responses, Histology and Gene Expression in Nile Tilapia, <i>Oreochromis niloticus</i>Seonghun Won0Ali Hamidoghli1Wonsuk Choi2Youngjin Park3Won Je Jang4In-Soo Kong5Sungchul C. Bai6Department of Marine Bio-materials and Aquaculture, Feeds and Foods Nutrition Research Center (FFNRC), Pukyong National University, Busan 608-737, KoreaDepartment of Marine Bio-materials and Aquaculture, Feeds and Foods Nutrition Research Center (FFNRC), Pukyong National University, Busan 608-737, KoreaDepartment of Marine Bio-materials and Aquaculture, Feeds and Foods Nutrition Research Center (FFNRC), Pukyong National University, Busan 608-737, KoreaFaculty of Biosciences and Aquaculture, Nord University, Universitetsallen 11, 8049 Bodo, NorwayDepartment of Biotechnology, Pukyong National University, Busan 608-737, KoreaDepartment of Biotechnology, Pukyong National University, Busan 608-737, KoreaDepartment of Marine Bio-materials and Aquaculture, Feeds and Foods Nutrition Research Center (FFNRC), Pukyong National University, Busan 608-737, KoreaAn eight-week feeding trial was conducted to evaluate the effects of a basal control diet (CON), <i>Bacillus subtilis</i> at 10<sup>7</sup> (BS<sub>7</sub>) and at 10<sup>8</sup> CFU/g diet (BS<sub>8</sub>), <i>Lactococcus lactis</i> at 10<sup>7</sup> CFU/g (LL<sub>7</sub>) and at 10<sup>8</sup> CFU/g diet (LL<sub>8</sub>), and oxytetracycline (OTC) at 4 g/kg diet on Nile tilapia. Fish with initial body weight of 2.83 ± 0.05 g (mean ± SD) were fed two times a day. Weight gain, specific growth rate, feed efficiency, protein efficiency ratio and lysozyme activity of fish fed BS<sub>8</sub>, LL<sub>8</sub> and LL<sub>7</sub> diets were significantly higher than those of fish fed CON diet (<i>p</i> < 0.05). Superoxide dismutase and myeloperoxidase activity of fish fed BS<sub>8</sub>, LL<sub>8,</sub> BS<sub>7</sub>, LL<sub>7</sub> and OTC diets were significantly higher than those of fish fed CON diet. Intestinal villi length and muscular layer thickness of fish fed BS<sub>8</sub>, LL<sub>8</sub> and LL<sub>7</sub> diets were significantly higher than those of fish fed CON and OTC diets. Also, heat shock protein 70 (HSP70), interleukin (IL-1β), interferon-gamma (IFN-γ) and tumour necrosis factor (TNF-α) gene expression of fish fed BS<sub>8</sub> and LL<sub>8</sub> diets were significantly higher than those of fish fed CON diet. After 13 days of challenge test, cumulative survival rate of fish fed BS<sub>8</sub> and LL<sub>8</sub> diets were significantly higher than those of fish fed CON, BS<sub>7</sub> and OTC diets. Based on these results, <i>B. subtilis</i> and <i>L. lactis</i> at 10<sup>8</sup> (CFU/g) could replace antibiotics, and have beneficial effects on growth, immunity, histology, gene expression, and disease resistance in Nile tilapia.https://www.mdpi.com/2076-2607/8/1/67probiotic<i>bacillus</i><i>lactococcus</i>nile tilapiagrowthimmunity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Seonghun Won Ali Hamidoghli Wonsuk Choi Youngjin Park Won Je Jang In-Soo Kong Sungchul C. Bai |
spellingShingle |
Seonghun Won Ali Hamidoghli Wonsuk Choi Youngjin Park Won Je Jang In-Soo Kong Sungchul C. Bai Effects of <i>Bacillus subtilis</i> WB60 and <i>Lactococcus lactis</i> on Growth, Immune Responses, Histology and Gene Expression in Nile Tilapia, <i>Oreochromis niloticus</i> Microorganisms probiotic <i>bacillus</i> <i>lactococcus</i> nile tilapia growth immunity |
author_facet |
Seonghun Won Ali Hamidoghli Wonsuk Choi Youngjin Park Won Je Jang In-Soo Kong Sungchul C. Bai |
author_sort |
Seonghun Won |
title |
Effects of <i>Bacillus subtilis</i> WB60 and <i>Lactococcus lactis</i> on Growth, Immune Responses, Histology and Gene Expression in Nile Tilapia, <i>Oreochromis niloticus</i> |
title_short |
Effects of <i>Bacillus subtilis</i> WB60 and <i>Lactococcus lactis</i> on Growth, Immune Responses, Histology and Gene Expression in Nile Tilapia, <i>Oreochromis niloticus</i> |
title_full |
Effects of <i>Bacillus subtilis</i> WB60 and <i>Lactococcus lactis</i> on Growth, Immune Responses, Histology and Gene Expression in Nile Tilapia, <i>Oreochromis niloticus</i> |
title_fullStr |
Effects of <i>Bacillus subtilis</i> WB60 and <i>Lactococcus lactis</i> on Growth, Immune Responses, Histology and Gene Expression in Nile Tilapia, <i>Oreochromis niloticus</i> |
title_full_unstemmed |
Effects of <i>Bacillus subtilis</i> WB60 and <i>Lactococcus lactis</i> on Growth, Immune Responses, Histology and Gene Expression in Nile Tilapia, <i>Oreochromis niloticus</i> |
title_sort |
effects of <i>bacillus subtilis</i> wb60 and <i>lactococcus lactis</i> on growth, immune responses, histology and gene expression in nile tilapia, <i>oreochromis niloticus</i> |
publisher |
MDPI AG |
series |
Microorganisms |
issn |
2076-2607 |
publishDate |
2020-01-01 |
description |
An eight-week feeding trial was conducted to evaluate the effects of a basal control diet (CON), <i>Bacillus subtilis</i> at 10<sup>7</sup> (BS<sub>7</sub>) and at 10<sup>8</sup> CFU/g diet (BS<sub>8</sub>), <i>Lactococcus lactis</i> at 10<sup>7</sup> CFU/g (LL<sub>7</sub>) and at 10<sup>8</sup> CFU/g diet (LL<sub>8</sub>), and oxytetracycline (OTC) at 4 g/kg diet on Nile tilapia. Fish with initial body weight of 2.83 ± 0.05 g (mean ± SD) were fed two times a day. Weight gain, specific growth rate, feed efficiency, protein efficiency ratio and lysozyme activity of fish fed BS<sub>8</sub>, LL<sub>8</sub> and LL<sub>7</sub> diets were significantly higher than those of fish fed CON diet (<i>p</i> < 0.05). Superoxide dismutase and myeloperoxidase activity of fish fed BS<sub>8</sub>, LL<sub>8,</sub> BS<sub>7</sub>, LL<sub>7</sub> and OTC diets were significantly higher than those of fish fed CON diet. Intestinal villi length and muscular layer thickness of fish fed BS<sub>8</sub>, LL<sub>8</sub> and LL<sub>7</sub> diets were significantly higher than those of fish fed CON and OTC diets. Also, heat shock protein 70 (HSP70), interleukin (IL-1β), interferon-gamma (IFN-γ) and tumour necrosis factor (TNF-α) gene expression of fish fed BS<sub>8</sub> and LL<sub>8</sub> diets were significantly higher than those of fish fed CON diet. After 13 days of challenge test, cumulative survival rate of fish fed BS<sub>8</sub> and LL<sub>8</sub> diets were significantly higher than those of fish fed CON, BS<sub>7</sub> and OTC diets. Based on these results, <i>B. subtilis</i> and <i>L. lactis</i> at 10<sup>8</sup> (CFU/g) could replace antibiotics, and have beneficial effects on growth, immunity, histology, gene expression, and disease resistance in Nile tilapia. |
topic |
probiotic <i>bacillus</i> <i>lactococcus</i> nile tilapia growth immunity |
url |
https://www.mdpi.com/2076-2607/8/1/67 |
work_keys_str_mv |
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