Immobilization Effects on the Catalytic Properties of Two Fusarium Verticillioides Lipases: Stability, Hydrolysis, Transesterification and Enantioselectivity Improvement

Immobilization Effects on the Catalytic Properties of Two Fusarium Verticillioides Lipases: Stability, Hydrolysis, Transesterification and Enantioselectivity Improvement

Fusarium verticillioides lipases were purified in a “cascade” method using octadecyl Sepabeads and octyl Sepharose resins, which led to the isolation of two proteins with lipolytic activities. Lip 1 was purified after octyl Sepharose adsorption presenting 30.3 kDa and, Lip 2 presented 68.0 kDa after...

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Bibliographic Details
Main Authors: Fernanda Dell Antonio Facchini, Marita Gimenez Pereira, Ana Claudia Vici, Marco Filice, Benevides Costa Pessela, Jose Manuel Guisan, Glória Fernandez-Lorente, Maria de Lourdes Teixeira de Moraes Polizeli
Format: Article
Language:English
Published: MDPI AG 2018-02-01
Series:Catalysts
Subjects:
immobilization
purification
lipase
Fusarium verticillioides
EPA
DHA
Online Access:http://www.mdpi.com/2073-4344/8/2/84

Internet

http://www.mdpi.com/2073-4344/8/2/84

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