Chicken Cytochrome P450 1A5 Is the Key Enzyme for Metabolizing T-2 Toxin to 3'OH-T-2

Chicken Cytochrome P450 1A5 Is the Key Enzyme for Metabolizing T-2 Toxin to 3'OH-T-2

The transmission of T-2 toxin and its metabolites into the edible tissues of poultry has potential effects on human health. We report that T-2 toxin significantly induces CYP1A4 and CYP1A5 expression in chicken embryonic hepatocyte cells. The enzyme activity assays of CYP1A4 and CYP1A5 heterologousl...

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Main Authors: Shufeng Shang, Jun Jiang, Yiqun Deng
Format: Article
Language:English
Published: MDPI AG 2013-05-01
Series:International Journal of Molecular Sciences
Subjects:
T-2 toxin
chicken CYP1A5
metabolism
Online Access:http://www.mdpi.com/1422-0067/14/6/10809
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spelling doaj-0bbe6bbbda3245c19542d6dbc429ce4f2020-11-24T21:04:43ZengMDPI AGInternational Journal of Molecular Sciences1422-00672013-05-01146108091081810.3390/ijms140610809Chicken Cytochrome P450 1A5 Is the Key Enzyme for Metabolizing T-2 Toxin to 3'OH-T-2Shufeng ShangJun JiangYiqun DengThe transmission of T-2 toxin and its metabolites into the edible tissues of poultry has potential effects on human health. We report that T-2 toxin significantly induces CYP1A4 and CYP1A5 expression in chicken embryonic hepatocyte cells. The enzyme activity assays of CYP1A4 and CYP1A5 heterologously expressed in HeLa cells indicate that only CYP1A5 metabolizes T-2 to 3'OH-T-2 by the 3'-hydroxylation of isovaleryl groups. In vitro enzyme assays of recombinant CYP1A5 expressed in DH5α further confirm that CYP1A5 can convert T-2 into TC-1 (3'OH-T-2). Therefore, CYP1A5 is critical for the metabolism of trichothecene mycotoxin in chickens.http://www.mdpi.com/1422-0067/14/6/10809T-2 toxinchicken CYP1A5metabolism
collection DOAJ
language English
format Article
sources DOAJ
author Shufeng Shang
Jun Jiang
Yiqun Deng
spellingShingle Shufeng Shang
Jun Jiang
Yiqun Deng
Chicken Cytochrome P450 1A5 Is the Key Enzyme for Metabolizing T-2 Toxin to 3'OH-T-2
International Journal of Molecular Sciences
T-2 toxin
chicken CYP1A5
metabolism
author_facet Shufeng Shang
Jun Jiang
Yiqun Deng
author_sort Shufeng Shang
title Chicken Cytochrome P450 1A5 Is the Key Enzyme for Metabolizing T-2 Toxin to 3'OH-T-2
title_short Chicken Cytochrome P450 1A5 Is the Key Enzyme for Metabolizing T-2 Toxin to 3'OH-T-2
title_full Chicken Cytochrome P450 1A5 Is the Key Enzyme for Metabolizing T-2 Toxin to 3'OH-T-2
title_fullStr Chicken Cytochrome P450 1A5 Is the Key Enzyme for Metabolizing T-2 Toxin to 3'OH-T-2
title_full_unstemmed Chicken Cytochrome P450 1A5 Is the Key Enzyme for Metabolizing T-2 Toxin to 3'OH-T-2
title_sort chicken cytochrome p450 1a5 is the key enzyme for metabolizing t-2 toxin to 3'oh-t-2
publisher MDPI AG
series International Journal of Molecular Sciences
issn 1422-0067
publishDate 2013-05-01
description The transmission of T-2 toxin and its metabolites into the edible tissues of poultry has potential effects on human health. We report that T-2 toxin significantly induces CYP1A4 and CYP1A5 expression in chicken embryonic hepatocyte cells. The enzyme activity assays of CYP1A4 and CYP1A5 heterologously expressed in HeLa cells indicate that only CYP1A5 metabolizes T-2 to 3'OH-T-2 by the 3'-hydroxylation of isovaleryl groups. In vitro enzyme assays of recombinant CYP1A5 expressed in DH5α further confirm that CYP1A5 can convert T-2 into TC-1 (3'OH-T-2). Therefore, CYP1A5 is critical for the metabolism of trichothecene mycotoxin in chickens.
topic T-2 toxin
chicken CYP1A5
metabolism
url http://www.mdpi.com/1422-0067/14/6/10809
work_keys_str_mv AT shufengshang chickencytochromep4501a5isthekeyenzymeformetabolizingt2toxinto3039oht2
AT junjiang chickencytochromep4501a5isthekeyenzymeformetabolizingt2toxinto3039oht2
AT yiqundeng chickencytochromep4501a5isthekeyenzymeformetabolizingt2toxinto3039oht2
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