Expression profile of genes regulated by activity of the Na-H exchanger NHE1

• Main Authors: Putney Luanna K, Barber Diane L
• Format: Article
• Language: English
• Published: BMC 2004-07-01
• Series: BMC Genomics
• Online Access: http://www.biomedcentral.com/1471-2164/5/46

<p>Abstract</p> <p>Background</p> <p>In mammalian cells changes in intracellular pH (pH_i), which are predominantly controlled by activity of plasma membrane ion exchangers, regulate a diverse range of normal and pathological cellular processes. How changes in pH_iaffect distinct cellular processes has primarily been determined by evaluating protein activities and we know little about how pH_iregulates gene expression.</p> <p>Results</p> <p>A global profile of genes regulated in mammalian fibroblasts by decreased pH_iinduced by impaired activity of the plasma membrane Na-H exchanger NHE1 was characterized by using cDNA microarrays. Analysis of selected genes by quantitative RT-PCR, TaqMan, and immunoblot analyses confirmed results obtained from cDNA arrays. Consistent with established roles of pH_iand NHE1 activity in cell proliferation and oncogenic transformation, grouping regulated genes into functional categories and biological pathways indicated a predominant number of genes with altered expression were associated with growth factor signaling, oncogenesis, and cell cycle progression.</p> <p>Conclusion</p> <p>A comprehensive analysis of genes selectively regulated by pH_iprovides insight on candidate targets that might mediate established effects of pH_ion a number of normal and pathological cell functions.</p>