STUDY ON IN VITRO PROPAGATION AND DEVELOPMENT GROWTH OF GYNOSTEMMA PUBESCENS IN THE GREENHOUSE CONDITION

The Gynostemma pubescens plant, which is distributed mainly over the northern provinces, is a valuable and beneficial herb for human health. In this study, we investigated in vitro propagation and cultivation to evaluate the growth and development of Gynostemma pubescens in greenhouse conditions at...

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Main Authors: Nguyễn Thị Thanh Hằng, Lê Ái Vân, Đinh Văn Khiêm, Hoàng Văn Cương, Nguyễn Thị Phượng Hoàng, Phan Xuân Huyên
Format: Article
Language:English
Published: Dalat University 2018-09-01
Series:Tạp chí Khoa học Đại học Đà Lạt
Subjects:
Online Access:http://tckh.dlu.edu.vn/index.php/tckhdhdl/article/view/506
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spelling doaj-0b888d1370c84e6780a5a916ffb475892020-11-25T03:54:20ZengDalat UniversityTạp chí Khoa học Đại học Đà Lạt0866-787X0866-787X2018-09-01839911210.37569/DalatUniversity.8.3.506(2018)261STUDY ON IN VITRO PROPAGATION AND DEVELOPMENT GROWTH OF GYNOSTEMMA PUBESCENS IN THE GREENHOUSE CONDITIONNguyễn Thị Thanh Hằng0Lê Ái Vân1Đinh Văn Khiêm2Hoàng Văn Cương3Nguyễn Thị Phượng Hoàng4Phan Xuân Huyên5Viện Nghiên cứu Khoa học Tây NguyênViện Nghiên cứu Khoa học Tây NguyênViện Nghiên cứu Khoa học Tây NguyênViện Nghiên cứu Khoa học Tây NguyênViện Nghiên cứu Khoa học Tây NguyênViện Nghiên cứu Khoa học Tây NguyênThe Gynostemma pubescens plant, which is distributed mainly over the northern provinces, is a valuable and beneficial herb for human health. In this study, we investigated in vitro propagation and cultivation to evaluate the growth and development of Gynostemma pubescens in greenhouse conditions at Dalat city, Lamdong province. The results showed that MS medium supplemented with 1mg/l BA, 30 g/l sucrose, 8g/l agar, pH 5.8 was the best medium for shoot regeneration in vitro, with a shoot height of 1.84cm, and 10.50 shoots/explant. The MS medium contained 0.5mg/l TDZ, 30g/l sucrose, 8g/l agar, pH 5.8 was the most suitable medium for shoot regeneration in vitro, with a shoot height of 1.99cm, and 13.80 shoots/explant. Concentrations of IBA from 0 to 1 mg/l were appropriate for in vitro root regeneration, yielding a root regeneration rate of 100%. Coconut fiber powder was the most suitable substrate to transfer the plantlets to the greenhouse, with a survival rate of 100%, plant height of 9.73cm, and root length of 6.45cm. Humus proved the best substrate for plant development and growth, with a survival rate of 100%, plant height of 82.08cm, root length of 36.57cm and a fresh weight of 57.32g/plant. Watering with 100 ml of Nitrophoska fertilizer (2g/l) once a week was the best for plant growth, with a survival rate of 100%, height of 94.22cm, root length of 37.71cm, and a fresh weight of 59.38g/plant.http://tckh.dlu.edu.vn/index.php/tckhdhdl/article/view/506chiều cao câychiều dài rễcây giảo cổ lamsinh trưởng phát triểnsố chồi.
collection DOAJ
language English
format Article
sources DOAJ
author Nguyễn Thị Thanh Hằng
Lê Ái Vân
Đinh Văn Khiêm
Hoàng Văn Cương
Nguyễn Thị Phượng Hoàng
Phan Xuân Huyên
spellingShingle Nguyễn Thị Thanh Hằng
Lê Ái Vân
Đinh Văn Khiêm
Hoàng Văn Cương
Nguyễn Thị Phượng Hoàng
Phan Xuân Huyên
STUDY ON IN VITRO PROPAGATION AND DEVELOPMENT GROWTH OF GYNOSTEMMA PUBESCENS IN THE GREENHOUSE CONDITION
Tạp chí Khoa học Đại học Đà Lạt
chiều cao cây
chiều dài rễ
cây giảo cổ lam
sinh trưởng phát triển
số chồi.
author_facet Nguyễn Thị Thanh Hằng
Lê Ái Vân
Đinh Văn Khiêm
Hoàng Văn Cương
Nguyễn Thị Phượng Hoàng
Phan Xuân Huyên
author_sort Nguyễn Thị Thanh Hằng
title STUDY ON IN VITRO PROPAGATION AND DEVELOPMENT GROWTH OF GYNOSTEMMA PUBESCENS IN THE GREENHOUSE CONDITION
title_short STUDY ON IN VITRO PROPAGATION AND DEVELOPMENT GROWTH OF GYNOSTEMMA PUBESCENS IN THE GREENHOUSE CONDITION
title_full STUDY ON IN VITRO PROPAGATION AND DEVELOPMENT GROWTH OF GYNOSTEMMA PUBESCENS IN THE GREENHOUSE CONDITION
title_fullStr STUDY ON IN VITRO PROPAGATION AND DEVELOPMENT GROWTH OF GYNOSTEMMA PUBESCENS IN THE GREENHOUSE CONDITION
title_full_unstemmed STUDY ON IN VITRO PROPAGATION AND DEVELOPMENT GROWTH OF GYNOSTEMMA PUBESCENS IN THE GREENHOUSE CONDITION
title_sort study on in vitro propagation and development growth of gynostemma pubescens in the greenhouse condition
publisher Dalat University
series Tạp chí Khoa học Đại học Đà Lạt
issn 0866-787X
0866-787X
publishDate 2018-09-01
description The Gynostemma pubescens plant, which is distributed mainly over the northern provinces, is a valuable and beneficial herb for human health. In this study, we investigated in vitro propagation and cultivation to evaluate the growth and development of Gynostemma pubescens in greenhouse conditions at Dalat city, Lamdong province. The results showed that MS medium supplemented with 1mg/l BA, 30 g/l sucrose, 8g/l agar, pH 5.8 was the best medium for shoot regeneration in vitro, with a shoot height of 1.84cm, and 10.50 shoots/explant. The MS medium contained 0.5mg/l TDZ, 30g/l sucrose, 8g/l agar, pH 5.8 was the most suitable medium for shoot regeneration in vitro, with a shoot height of 1.99cm, and 13.80 shoots/explant. Concentrations of IBA from 0 to 1 mg/l were appropriate for in vitro root regeneration, yielding a root regeneration rate of 100%. Coconut fiber powder was the most suitable substrate to transfer the plantlets to the greenhouse, with a survival rate of 100%, plant height of 9.73cm, and root length of 6.45cm. Humus proved the best substrate for plant development and growth, with a survival rate of 100%, plant height of 82.08cm, root length of 36.57cm and a fresh weight of 57.32g/plant. Watering with 100 ml of Nitrophoska fertilizer (2g/l) once a week was the best for plant growth, with a survival rate of 100%, height of 94.22cm, root length of 37.71cm, and a fresh weight of 59.38g/plant.
topic chiều cao cây
chiều dài rễ
cây giảo cổ lam
sinh trưởng phát triển
số chồi.
url http://tckh.dlu.edu.vn/index.php/tckhdhdl/article/view/506
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