In vitro antibacterial activity and in vivo therapeutic effect of Sesbania grandiflora in bacterial infected silkworms

Context: Antibiotic resistance is a serious problem worldwide. Searching for new potential agents is, therefore, essential. The bark of Sesbania grandiflora (L.) Pers. (Fabaceae) has been used in folk medicine against various diseases. Objective: To investigate the antibacterial activity of S. grand...

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Main Authors: Pimporn Anantaworasakul, Hiroshi Hamamoto, Kazuhisa Sekimizu, Siriporn Okonogi
Format: Article
Language:English
Published: Taylor & Francis Group 2017-01-01
Series:Pharmaceutical Biology
Subjects:
Online Access:http://dx.doi.org/10.1080/13880209.2017.1297467
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spelling doaj-0b84bc7c94694c3993c12480f158c6bf2020-11-25T03:33:52ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162017-01-015511256126210.1080/13880209.2017.12974671297467In vitro antibacterial activity and in vivo therapeutic effect of Sesbania grandiflora in bacterial infected silkwormsPimporn Anantaworasakul0Hiroshi Hamamoto1Kazuhisa Sekimizu2Siriporn Okonogi3Chiang Mai UniversityTeikyo UniversityTeikyo UniversityChiang Mai UniversityContext: Antibiotic resistance is a serious problem worldwide. Searching for new potential agents is, therefore, essential. The bark of Sesbania grandiflora (L.) Pers. (Fabaceae) has been used in folk medicine against various diseases. Objective: To investigate the antibacterial activity of S. grandiflora bark and explore the therapeutic effect of the highest potent fraction. Materials and methods: Bacteria and healthy silkworms were exposed to three fractionated extracts (3.1–400 mg/mL) of S. grandiflora bark from hexane (HXF), chloroform (CFF), and ethyl acetate (EAF). The sets of bacteria were incubated at 37 °C while silkworms were kept at 27 °C for 24 h. To evaluate the therapeutic effect, silkworms infected with bacteria were exposed to the extracts (0.5–60 mg/mL) and incubated at 27 °C for 52 h. Qualitative analysis of the most potent extract was done using HPLC. Results: EAF showed the highest activity with MIC against methicillin resistant Staphylococcus aureus (MRSA) and vancomycin resistant enterococci (VRE) of 1.6 and 0.4 mg/mL, respectively, and against Gram-negative Escherichia coli and Pseudomonas aeruginosa of 6.2 and 3.1 mg/mL, respectively. It is nontoxic to silkworms with LC50 >400 mg/mL and has high therapeutic effect on infected silkworms with EC50 of 1.9 mg/mL. EAF consists of at least five major compounds, one of them is gallic acid. The activity of EAF is higher than the sum of individual activities of separated compounds. Discussion and conclusion: These results suggest that EAF is a promising antibacterial extract, suitable for further investigation in rodents infected with drug resistant bacteria.http://dx.doi.org/10.1080/13880209.2017.1297467antibacterial extractdrug-resistant organismssilkworm infectionkilling rate
collection DOAJ
language English
format Article
sources DOAJ
author Pimporn Anantaworasakul
Hiroshi Hamamoto
Kazuhisa Sekimizu
Siriporn Okonogi
spellingShingle Pimporn Anantaworasakul
Hiroshi Hamamoto
Kazuhisa Sekimizu
Siriporn Okonogi
In vitro antibacterial activity and in vivo therapeutic effect of Sesbania grandiflora in bacterial infected silkworms
Pharmaceutical Biology
antibacterial extract
drug-resistant organisms
silkworm infection
killing rate
author_facet Pimporn Anantaworasakul
Hiroshi Hamamoto
Kazuhisa Sekimizu
Siriporn Okonogi
author_sort Pimporn Anantaworasakul
title In vitro antibacterial activity and in vivo therapeutic effect of Sesbania grandiflora in bacterial infected silkworms
title_short In vitro antibacterial activity and in vivo therapeutic effect of Sesbania grandiflora in bacterial infected silkworms
title_full In vitro antibacterial activity and in vivo therapeutic effect of Sesbania grandiflora in bacterial infected silkworms
title_fullStr In vitro antibacterial activity and in vivo therapeutic effect of Sesbania grandiflora in bacterial infected silkworms
title_full_unstemmed In vitro antibacterial activity and in vivo therapeutic effect of Sesbania grandiflora in bacterial infected silkworms
title_sort in vitro antibacterial activity and in vivo therapeutic effect of sesbania grandiflora in bacterial infected silkworms
publisher Taylor & Francis Group
series Pharmaceutical Biology
issn 1388-0209
1744-5116
publishDate 2017-01-01
description Context: Antibiotic resistance is a serious problem worldwide. Searching for new potential agents is, therefore, essential. The bark of Sesbania grandiflora (L.) Pers. (Fabaceae) has been used in folk medicine against various diseases. Objective: To investigate the antibacterial activity of S. grandiflora bark and explore the therapeutic effect of the highest potent fraction. Materials and methods: Bacteria and healthy silkworms were exposed to three fractionated extracts (3.1–400 mg/mL) of S. grandiflora bark from hexane (HXF), chloroform (CFF), and ethyl acetate (EAF). The sets of bacteria were incubated at 37 °C while silkworms were kept at 27 °C for 24 h. To evaluate the therapeutic effect, silkworms infected with bacteria were exposed to the extracts (0.5–60 mg/mL) and incubated at 27 °C for 52 h. Qualitative analysis of the most potent extract was done using HPLC. Results: EAF showed the highest activity with MIC against methicillin resistant Staphylococcus aureus (MRSA) and vancomycin resistant enterococci (VRE) of 1.6 and 0.4 mg/mL, respectively, and against Gram-negative Escherichia coli and Pseudomonas aeruginosa of 6.2 and 3.1 mg/mL, respectively. It is nontoxic to silkworms with LC50 >400 mg/mL and has high therapeutic effect on infected silkworms with EC50 of 1.9 mg/mL. EAF consists of at least five major compounds, one of them is gallic acid. The activity of EAF is higher than the sum of individual activities of separated compounds. Discussion and conclusion: These results suggest that EAF is a promising antibacterial extract, suitable for further investigation in rodents infected with drug resistant bacteria.
topic antibacterial extract
drug-resistant organisms
silkworm infection
killing rate
url http://dx.doi.org/10.1080/13880209.2017.1297467
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