| Summary: | In this article the potential variation in biochemical constituents of peanut plant leaves affect by presoaking peanut seeds in copper oxide nanoparticles suspension has been studied and compared with its bulk counterpart. The synthesized nanoparticles were characterized by x-ray diffraction (XRD), scanning electron microscope (SEM) and transmission electron microscope (TEM) studies. The Fourier transform infrared (FTIR) analysis shows the most prominent peaks at ∼2923 cm−1, ∼1636 cm−1 and ∼1033 cm−1, which correspond to lipids, protein and carbohydrate content in leaf samples respectively. The calculated mean ratio of the peak intensities for various frequency regions and total band area calculation for various band regions explain the variation in lipid, protein and carbohydrate content of leaf samples. Further the FTIR spectra were processed by de-convolution and curve fitting to quantitatively examine the chemical contents and structure changing of the secondary structure of protein. The calculated integrated band area of β – sheet, β – turn and α – helix secondary structure of protein varies to greater extent in all samples compared to control. Principal component analysis (PCA) has been carried out to explain the total variance in secondary structure of protein content in peanut plant leaves. Principal component 1 (PC1) accounts for 63.50% variation in secondary structure of protein whereas principal component 2 (PC2) accounts for 29.56%. The application of nanoparticles via presoaking method implies potential variation in biochemical constituents but doesn't affect the growth of plants considerably.
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