Astragaloside IV Inhibits the Up-Regulation of Wnt/β-Catenin Signaling in Rats with Unilateral Ureteral Obstruction

• Main Authors: Li Wang, Yang-Feng Chi, Ze-Ting Yuan, Wen-Chao Zhou, Pei-Hao Yin, Xue-Mei Zhang, Wen Peng
• Format: Article
• Language: English
• Published: Cell Physiol Biochem Press GmbH & Co KG 2014-04-01
• Series: Cellular Physiology and Biochemistry
• Subjects: UUO model; Renal interstitial fibrosis; Astragaloside IV; Wnt/β-catenin signaling pathway
• Online Access: http://www.karger.com/Article/FullText/358699
• ISSN: 1015-8987; 1421-9778

Objective: To investigate the effect of Astragaloside IV (AS-IV) on the regulation of the Wnt/β-catenin signaling pathway in rats with unilateral ureteral obstruction (UUO). Methods: Rat renal interstitial fibrosis models were prepared using unilateral ureteral ligation. Rats were randomly divided into sham group, sham group with AS-IV (33mg/kg), unilateral ureteral obstruction group, and unilateral ureteral obstruction group receiving varied doses of AS-IV (3.3, 10, and 33 mg/kg). Immunohistochemical analysis, real-time fluorescence quantitative PCR (FQ-PCR), and western blot were used to detect the expression of genes and proteins associated with the Wnt/β-catenin signaling pathway in renal tissues. Results: Levels of Wnt3, Wnt4, and Frizzled gene expression increased significantly in the UUO model; AS-IV was associated with the downregulation of the expression of Wnt3, Wnt4, Frizzled4, p-LRP5, p-LRP6, disheveled, β-catenin, LEF-1, TCF-1, Snail, Jagged 1, Twist, MMP2, and MMP7 proteins in a concentration-dependent manner, while the expression of APC, CK1, and E-cadherin was increased. Conclusions: AS-IV effectively inhibits the up-regulation of proteins in the Wnt/β-catenin signaling pathway in UUO-model rats, indicating its possible inhibitory effects on renal interstitial fibrosis.