Formononetin Attenuates IL-1β-Induced Apoptosis and NF-κB Activation in INS-1 Cells

Several studies suggest that the inflammation plays a role in the pathogenesis of some glucose disorders in adults. Exposure of pancreatic β-cells to cytokines, such as interleukin-1β (IL-1β), is thought to contribute to β-cell apoptosis. One important event triggered by IL-1β is induction of nitric...

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Main Authors: Xiao Han, Zhengqiu Zhu, Dong Wang, Zuoling Xie, Han Yin, Yunxia Zhu, Lu Gao, Yao Wang
Format: Article
Language:English
Published: MDPI AG 2012-08-01
Series:Molecules
Subjects:
Online Access:http://www.mdpi.com/1420-3049/17/9/10052
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spelling doaj-0b5f96a31f474952808c596a6202aead2020-11-24T23:18:50ZengMDPI AGMolecules1420-30492012-08-01179100521006410.3390/molecules170910052Formononetin Attenuates IL-1β-Induced Apoptosis and NF-κB Activation in INS-1 CellsXiao HanZhengqiu ZhuDong WangZuoling XieHan YinYunxia ZhuLu GaoYao WangSeveral studies suggest that the inflammation plays a role in the pathogenesis of some glucose disorders in adults. Exposure of pancreatic β-cells to cytokines, such as interleukin-1β (IL-1β), is thought to contribute to β-cell apoptosis. One important event triggered by IL-1β is induction of nitric oxide synthase (iNOS), an enzyme that catalyzes intracellular generation of the cytotoxic free radical NO. Recent work have suggested that formononetin, as an <em>O</em>-methylated isoflavone found in a number of plants and herbs like <em>Astragalus membranaceus</em>, inhibited some pro-inflammatory cytokine production in macrophages. However, the roles of formononetin in pancreatic beta cells have not been fully established. The aim of the present study was to assess possible <em>in vitro</em> effects of formononetin on cell apoptosis induced by IL-1β in the rat insulinoma cell line, INS-1. Our results demonstrate that formononetin significantly prevents IL-1β-increased INS-1 cell death and blocks cytokine-induced apoptotic signaling (the reduction of Bax/Bcl-2 ratio and caspase-3 activity). Formononetin also inhibited the activation of nuclear factor-kappaB (NF-κB), which is a significant transcription factor for iNOS, so as to decease nitric oxide (NO) formation in a dose dependent manner <em>in vitro</em>. Our observations indicated that formononetin could protect against pancreatic β-cell apoptosis caused by IL-1β and therefore could be used in the future as a new drug improving diabetes mellitus.http://www.mdpi.com/1420-3049/17/9/10052<em>INS-1</em>NF-κBiNOSformononetinapoptosis
collection DOAJ
language English
format Article
sources DOAJ
author Xiao Han
Zhengqiu Zhu
Dong Wang
Zuoling Xie
Han Yin
Yunxia Zhu
Lu Gao
Yao Wang
spellingShingle Xiao Han
Zhengqiu Zhu
Dong Wang
Zuoling Xie
Han Yin
Yunxia Zhu
Lu Gao
Yao Wang
Formononetin Attenuates IL-1β-Induced Apoptosis and NF-κB Activation in INS-1 Cells
Molecules
<em>INS-1</em>
NF-κB
iNOS
formononetin
apoptosis
author_facet Xiao Han
Zhengqiu Zhu
Dong Wang
Zuoling Xie
Han Yin
Yunxia Zhu
Lu Gao
Yao Wang
author_sort Xiao Han
title Formononetin Attenuates IL-1β-Induced Apoptosis and NF-κB Activation in INS-1 Cells
title_short Formononetin Attenuates IL-1β-Induced Apoptosis and NF-κB Activation in INS-1 Cells
title_full Formononetin Attenuates IL-1β-Induced Apoptosis and NF-κB Activation in INS-1 Cells
title_fullStr Formononetin Attenuates IL-1β-Induced Apoptosis and NF-κB Activation in INS-1 Cells
title_full_unstemmed Formononetin Attenuates IL-1β-Induced Apoptosis and NF-κB Activation in INS-1 Cells
title_sort formononetin attenuates il-1β-induced apoptosis and nf-κb activation in ins-1 cells
publisher MDPI AG
series Molecules
issn 1420-3049
publishDate 2012-08-01
description Several studies suggest that the inflammation plays a role in the pathogenesis of some glucose disorders in adults. Exposure of pancreatic β-cells to cytokines, such as interleukin-1β (IL-1β), is thought to contribute to β-cell apoptosis. One important event triggered by IL-1β is induction of nitric oxide synthase (iNOS), an enzyme that catalyzes intracellular generation of the cytotoxic free radical NO. Recent work have suggested that formononetin, as an <em>O</em>-methylated isoflavone found in a number of plants and herbs like <em>Astragalus membranaceus</em>, inhibited some pro-inflammatory cytokine production in macrophages. However, the roles of formononetin in pancreatic beta cells have not been fully established. The aim of the present study was to assess possible <em>in vitro</em> effects of formononetin on cell apoptosis induced by IL-1β in the rat insulinoma cell line, INS-1. Our results demonstrate that formononetin significantly prevents IL-1β-increased INS-1 cell death and blocks cytokine-induced apoptotic signaling (the reduction of Bax/Bcl-2 ratio and caspase-3 activity). Formononetin also inhibited the activation of nuclear factor-kappaB (NF-κB), which is a significant transcription factor for iNOS, so as to decease nitric oxide (NO) formation in a dose dependent manner <em>in vitro</em>. Our observations indicated that formononetin could protect against pancreatic β-cell apoptosis caused by IL-1β and therefore could be used in the future as a new drug improving diabetes mellitus.
topic <em>INS-1</em>
NF-κB
iNOS
formononetin
apoptosis
url http://www.mdpi.com/1420-3049/17/9/10052
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