Identification of chloroquine resistance Pfcrt-K76T and determination of Pfmdr1-N86Y copy number by SYBR Green I qPCR

Identification of chloroquine resistance Pfcrt-K76T and determination of Pfmdr1-N86Y copy number by SYBR Green I qPCR

Objective: To identify prevalence of chloroquine resistance point mutation at (Pfcrt, K76T) and (Pfmdr1, N86Y) copy number variation. Methods: SYBR Green I based real time PCR was used. One hundred and thirty-three samples were analyzed for (Pfcrt, K76T) and (Pfmdr1, N86Y) copy number from dried blo...

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Bibliographic Details
Main Authors: Addimas Tajebe, Mulugeta Aemero, Kimani Francis, Gabriel Magoma
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2015-03-01
Series:Asian Pacific Journal of Tropical Biomedicine
Subjects:
Plasmodium falciparum
DNA copy number variation
Pfmdr1
Pfcrt
Real-time PCR
Online Access:http://www.sciencedirect.com/science/article/pii/S2221169115300083

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