Hypoxia and oxidative stress markers in pediatric patients undergoing hemodialysis: cross section study

• Main Authors: Hamed Enas A, El-Abaseri Taghrid B, Mohamed Amany O, Ahmed Ahmed R, El-Metwally Tarek H
• Format: Article
• Language: English
• Published: BMC 2012-10-01
• Series: BMC Nephrology
• Online Access: http://www.biomedcentral.com/1471-2369/13/136

<p>Abstract</p> <p>Background</p> <p>Tissue injury due to hypoxia and/or free radicals is common in a variety of disease processes. This cross-sectional study aimed to investigate effect of chronic kidney diseases (CKD) and hemodialysis (HD) on hypoxia and oxidative stress biomarkers.</p> <p>Methods</p> <p>Forty pediatric patients with CKD on HD and 20 healthy children were recruited. Plasma hypoxia induced factor-1α (HIF-1α), vascular endothelial growth factor (VEGF) were measured by specific ELISA kits while, total antioxidant capacity (TAC), total peroxide (TPX), pyruvate and lactate by enzymatic/chemical colorimetric methods. Oxidative stress index (OSI) and lactate/pyruvate (L/P) ratio were calculated.</p> <p>Results</p> <p>TAC was significantly lower while TPX, OSI and VEGF were higher in patients at before- and after-dialysis session than controls. Lactate and HIF-1α levels were significantly higher at before-dialysis session than controls. Before dialysis, TAC and L/P ratio were lower than after-dialysis. In before-dialysis session, VEGF correlated positively with pyruvate, HIF-1α and OSI correlated positively with TPX, but, negatively with TAC. In after-dialysis session, HIF-1α correlated negatively with TPX and OSI; while, OSI correlated positively with TPX.</p> <p>Conclusions</p> <p>CKD patients succumb considerable tissue hypoxia with oxidative stress. Hemodialysis ameliorated hypoxia but lowered antioxidants as evidenced by decreased levels of HIF-1α and TAC at before- compared to after-dialysis levels.</p>