An integration-free iPSC line (SDQLCHi012-A) derived from a patient with inflammatory bowel disease- 28 carrying compound heterozygote mutations in IL10RA gene

An integration-free iPSC line (SDQLCHi012-A) derived from a patient with inflammatory bowel disease- 28 carrying compound heterozygote mutations in IL10RA gene

Induced pluripotent stem cell (iPSC) line (SDQLCHi012-A) was generated from peripheral blood mononuclear cells of an 11-month-old male who was diagnosed as inflammatory bowel disease-28 caused by compound heterozygote for IL10RA mutations (c.188 + 1G > A and c.301C > T). Non-integrating episom...

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Main Authors: Yanyan Ma, Haiyan Zhang, Shisong Zhang, Rui Dong, Xiaomeng Yang, Yue Li, Jingyun Guan, Zhongtao Gai, Yi Liu
Format: Article
Language:English
Published: Elsevier 2019-12-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506119302077
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spelling doaj-0b1a15b5a0a74db191270efef5ef17142020-11-25T02:44:09ZengElsevierStem Cell Research1873-50612019-12-0141An integration-free iPSC line (SDQLCHi012-A) derived from a patient with inflammatory bowel disease- 28 carrying compound heterozygote mutations in IL10RA geneYanyan Ma0Haiyan Zhang1Shisong Zhang2Rui Dong3Xiaomeng Yang4Yue Li5Jingyun Guan6Zhongtao Gai7Yi Liu8Pediatric Research Institute, Qilu Children's Hospital of Shandong University, Jinan, Shandong 250022, ChinaPediatric Research Institute, Qilu Children's Hospital of Shandong University, Jinan, Shandong 250022, ChinaGeneral Surgical Department, Qilu Children's Hospital of Shandong University, Jinan, Shandong 250022, ChinaPediatric Research Institute, Qilu Children's Hospital of Shandong University, Jinan, Shandong 250022, ChinaPediatric Research Institute, Qilu Children's Hospital of Shandong University, Jinan, Shandong 250022, ChinaPediatric Research Institute, Qilu Children's Hospital of Shandong University, Jinan, Shandong 250022, ChinaPediatric Research Institute, Qilu Children's Hospital of Shandong University, Jinan, Shandong 250022, ChinaCorresponding authors.; Pediatric Research Institute, Qilu Children's Hospital of Shandong University, Jinan, Shandong 250022, ChinaCorresponding authors.; Pediatric Research Institute, Qilu Children's Hospital of Shandong University, Jinan, Shandong 250022, ChinaInduced pluripotent stem cell (iPSC) line (SDQLCHi012-A) was generated from peripheral blood mononuclear cells of an 11-month-old male who was diagnosed as inflammatory bowel disease-28 caused by compound heterozygote for IL10RA mutations (c.188 + 1G > A and c.301C > T). Non-integrating episomal vectors coding OCT4, SOX2, KLF4, BCL-XL and MYC were used for reprogramming. The established iPSC line contained the same mutations identified in the patient, showed a normal karyotype, differentiation potential in vitro and expressed pluripotency markers.http://www.sciencedirect.com/science/article/pii/S1873506119302077
collection DOAJ
language English
format Article
sources DOAJ
author Yanyan Ma
Haiyan Zhang
Shisong Zhang
Rui Dong
Xiaomeng Yang
Yue Li
Jingyun Guan
Zhongtao Gai
Yi Liu
spellingShingle Yanyan Ma
Haiyan Zhang
Shisong Zhang
Rui Dong
Xiaomeng Yang
Yue Li
Jingyun Guan
Zhongtao Gai
Yi Liu
An integration-free iPSC line (SDQLCHi012-A) derived from a patient with inflammatory bowel disease- 28 carrying compound heterozygote mutations in IL10RA gene
Stem Cell Research
author_facet Yanyan Ma
Haiyan Zhang
Shisong Zhang
Rui Dong
Xiaomeng Yang
Yue Li
Jingyun Guan
Zhongtao Gai
Yi Liu
author_sort Yanyan Ma
title An integration-free iPSC line (SDQLCHi012-A) derived from a patient with inflammatory bowel disease- 28 carrying compound heterozygote mutations in IL10RA gene
title_short An integration-free iPSC line (SDQLCHi012-A) derived from a patient with inflammatory bowel disease- 28 carrying compound heterozygote mutations in IL10RA gene
title_full An integration-free iPSC line (SDQLCHi012-A) derived from a patient with inflammatory bowel disease- 28 carrying compound heterozygote mutations in IL10RA gene
title_fullStr An integration-free iPSC line (SDQLCHi012-A) derived from a patient with inflammatory bowel disease- 28 carrying compound heterozygote mutations in IL10RA gene
title_full_unstemmed An integration-free iPSC line (SDQLCHi012-A) derived from a patient with inflammatory bowel disease- 28 carrying compound heterozygote mutations in IL10RA gene
title_sort integration-free ipsc line (sdqlchi012-a) derived from a patient with inflammatory bowel disease- 28 carrying compound heterozygote mutations in il10ra gene
publisher Elsevier
series Stem Cell Research
issn 1873-5061
publishDate 2019-12-01
description Induced pluripotent stem cell (iPSC) line (SDQLCHi012-A) was generated from peripheral blood mononuclear cells of an 11-month-old male who was diagnosed as inflammatory bowel disease-28 caused by compound heterozygote for IL10RA mutations (c.188 + 1G > A and c.301C > T). Non-integrating episomal vectors coding OCT4, SOX2, KLF4, BCL-XL and MYC were used for reprogramming. The established iPSC line contained the same mutations identified in the patient, showed a normal karyotype, differentiation potential in vitro and expressed pluripotency markers.
url http://www.sciencedirect.com/science/article/pii/S1873506119302077
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