Intensification of microbial exopolysaccharide ethapolan synthesis under Acinetobacter sp. IМV B-7005 cultivation on sunflower oil
Introduction.Microbial exopolysaccharides (EPS) by the ability of their solutions to change the rheological properties of aqueous systems are widely used in various industries. In recent years, research on the use of industrial waste (including oil-containing) to obtain practically...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
National university of food technologies
2015-05-01
|
| Series: | Ukrainian Food Journal |
| Subjects: | |
| Online Access: | http://nbuv.gov.ua/j-pdf/UFJ_2014_3_2_14.pdf |
| Summary: | Introduction.Microbial exopolysaccharides (EPS) by the
ability of their solutions to change the rheological properties
of aqueous systems are widely used in various industries. In
recent years, research on the use of industrial waste
(including oil-containing) to obtain practically valuable
microbial metabolites intensified.
Materials and methods.Cultivation of Acinetobactersp.
IМV B-7005 strain was performed in liquid medium,
containing as a carbon source sunflower oil (1−5 %, v/v), a
source of nitrogen – ammonium nitrate (0.4−0.8 g/l), a
source of pantothenate − multivitamin complex
«Complevit» (0.00085 and0.00095 %). EPSconcentration
was determined gravimetrically after precipitation with
isopropanol, EPS-synthesizing ability − as a ratio of EPS
concentration to biomass concentration, wich was
expressed as g EPS / g biomass.
Results and discussions. It was established that
increasing the concentration of sunflower oil in basic
medium for Acinetobacter sp. IMV B -7005 cultivation to
4−5% was accompanied by decrease of ethapolan synthesis
compared with those in the medium containing lower (2−3
%) substrate concentration. Increasing ammonium nitrate
content to 0.6 g/l and/or pantothenate concentration to
0.00095% in a medium with 5% sunflower oil allowed to
increase the amount of ethapolan synthesized up to 6.6−6.7
g/l, that is in 1.3−1.4 times higher than in the basic medium
with the same concentration of the substrate but lower
NH4NO3 (0.4 g/l) and pantothenate (0.00085 %).
Conclusion. The obtained results indicate the possibility
of microbial polysaccharide ethapolan synthesis under
Acinetobacter sp. ІMV B -7005 cultivation in the medium
with a high content of sunflower oil. These data are the
basis for the development of ethapolan technology using as
a substrate fried oil. |
|---|---|
| ISSN: | 2304-974X 2313-5891 |