Development of a skeleton-specific antibody and Au nanoparticle-based immunochromatographic sensor for simultaneous detection of various tadalafil adulterants in health food

• Main Authors: Fan He, Tingting Zou, Jinyi Yang, Hong Wang, Lihua Deng, Yuanxin Tian, Zhenlin Xu, Yuanming Sun, Hongtao Lei, Xuecai Tan, Yudong Shen
• Format: Article
• Language: English
• Published: Taylor & Francis Group 2019-01-01
• Series: Food and Agricultural Immunology
• Subjects: tadalafil drugs; polyclonal antibodies; au nanoparticles; immunochromatographic sensor
• Online Access: http://dx.doi.org/10.1080/09540105.2019.1585417
• ISSN: 0954-0105; 1465-3443

In this work, a rapid, convenient and sensitive Au nanoparticle-based immunochromatographic sensor (AuNPs-ICS) for simultaneous detection of various tadalafil adulterants in health food was established for the first time, based on one high affinity and broad-spectrum antibody through skeleton-specific hapten design according to the common structure of tadalafil drugs. This immunochromatographic sensor was developed by the polyclonal antibody conjugated with AuNPs and the IC50 was 10.04 ng mL−1 for tadalafil and 9.55, 11.46, 17.01 ng mL−1 for amino tadalafil, acetamino tadalafil and nortadalafil, respectively. The limit of detection results could be obtained visually by naked eyes for and computationally by the hand-held immunochromatography reader. The sensor could specially detect Tadalafil and its analogs within 10 min in health food samples, and the close correlation with UPLC-MS/MS method showed the sensor was accurate and reliable, which could apply to on site detection Tadalafil and its analogs rapidly and accurately.