Analysis and Optimization of Conditions for the Use of 2′,7′-Dichlorofluorescein Diacetate in Cultured Hepatocytes

Analysis and Optimization of Conditions for the Use of 2′,7′-Dichlorofluorescein Diacetate in Cultured Hepatocytes

Numerous liver pathologies encompass oxidative stress as molecular basis of disease. The use of 2′,7′-dichlorodihydrofluorescein-diacetate (DCFH<sub>2</sub>-DA) as fluorogenic redox probe is problematic in liver cell lines because of membrane transport proteins that interfere with probe...

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Bibliographic Details
Main Authors: Megan J. Reiniers, Lianne R. de Haan, Laurens F. Reeskamp, Mans Broekgaarden, Rowan F. van Golen, Michal Heger
Format: Article
Language:English
Published: MDPI AG 2021-04-01
Series:Antioxidants
Subjects:
fluorogenic redox probe
oxidative and nitrosative stress
liver diseases
hepatocytes
cellular uptake and export
intravital fluorescence imaging
Online Access:https://www.mdpi.com/2076-3921/10/5/674

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