PERUBAHAN VIABILITAS DAN STRUKTUR SUBSELULER SPERMATOZOA DOMBA SETELAH PENGERINGBEKUAN

• Main Authors: Takdir Saili, I Ketut Mudite Adnyana, Ronny Rachman Noor, Mohamad Agus Setiadi, Srihadi Agungpriyono, Arief Boediono
• Format: Article
• Language: English
• Published: Universitas Udayana 2009-12-01
• Series: Jurnal Veteriner
• Subjects: freeze drying, ram spermatozoa, plasma membrane, acrosome cap
• Online Access: http://ojs.unud.ac.id/index.php/jvet/article/view/3368
• ISSN: 1411-8327; 2477-5665

<em style="color: #29363d; font-family: Tahoma, Arial, Helvetica, sans-serif; font-size: 14px; text-align: justify; background-color: #ddeaf4;">Several methods i.e. cooling, freezing, and freeze-drying have been widely used to preserve spermatozoa with various degree of success. Freeze-drying appears to provide a method to preserve spermatozoa in a dry state without requiring liquid nitrogen for storing frozen spermatozoa. Freeze-drying procedures can have a detrimental effect on plasma membrane and acrosomal cap of the spermatozoa. In this experiment study, the viability and subcellular changes of freeze-dried ram spermatozoa were evaluated using staining method and scanning electron microscopy. The results revealed that all freeze-dried spermatozoa were dead following evaluation using eosin staining and Hoechst-propidium iodide staining methods. Morover, plasma membrane and acrosomal cap of freeze-dried ram spermatozoa was disrupted observed using scanning electron microscope.</em>