Transgenic zebrafish recapitulating tbx16 gene early developmental expression.

Transgenic zebrafish recapitulating tbx16 gene early developmental expression.

We describe the creation of a transgenic zebrafish expressing GFP driven by a 7.5 kb promoter region of the tbx16 gene. This promoter segment is sufficient to recapitulate early embryonic expression of endogenous tbx16 in the presomitic mesoderm, the polster and, subsequently, in the hatching gland....

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Main Authors: Simon Wells, Svanhild Nornes, Michael Lardelli
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3123366?pdf=render
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spelling doaj-0ab87e69e98748cb9058214ac05c9da92020-11-25T02:31:04ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0166e2155910.1371/journal.pone.0021559Transgenic zebrafish recapitulating tbx16 gene early developmental expression.Simon WellsSvanhild NornesMichael LardelliWe describe the creation of a transgenic zebrafish expressing GFP driven by a 7.5 kb promoter region of the tbx16 gene. This promoter segment is sufficient to recapitulate early embryonic expression of endogenous tbx16 in the presomitic mesoderm, the polster and, subsequently, in the hatching gland. Expression of GFP in the transgenic lines later in development diverges to some extent from endogenous tbx16 expression with the serendipitous result that one line expresses GFP specifically in commissural primary ascending (CoPA) interneurons of the developing spinal cord. Using this line we demonstrate that the gene mafba (valentino) is expressed in CoPA interneurons.http://europepmc.org/articles/PMC3123366?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Simon Wells
Svanhild Nornes
Michael Lardelli
spellingShingle Simon Wells
Svanhild Nornes
Michael Lardelli
Transgenic zebrafish recapitulating tbx16 gene early developmental expression.
PLoS ONE
author_facet Simon Wells
Svanhild Nornes
Michael Lardelli
author_sort Simon Wells
title Transgenic zebrafish recapitulating tbx16 gene early developmental expression.
title_short Transgenic zebrafish recapitulating tbx16 gene early developmental expression.
title_full Transgenic zebrafish recapitulating tbx16 gene early developmental expression.
title_fullStr Transgenic zebrafish recapitulating tbx16 gene early developmental expression.
title_full_unstemmed Transgenic zebrafish recapitulating tbx16 gene early developmental expression.
title_sort transgenic zebrafish recapitulating tbx16 gene early developmental expression.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2011-01-01
description We describe the creation of a transgenic zebrafish expressing GFP driven by a 7.5 kb promoter region of the tbx16 gene. This promoter segment is sufficient to recapitulate early embryonic expression of endogenous tbx16 in the presomitic mesoderm, the polster and, subsequently, in the hatching gland. Expression of GFP in the transgenic lines later in development diverges to some extent from endogenous tbx16 expression with the serendipitous result that one line expresses GFP specifically in commissural primary ascending (CoPA) interneurons of the developing spinal cord. Using this line we demonstrate that the gene mafba (valentino) is expressed in CoPA interneurons.
url http://europepmc.org/articles/PMC3123366?pdf=render
work_keys_str_mv AT simonwells transgeniczebrafishrecapitulatingtbx16geneearlydevelopmentalexpression
AT svanhildnornes transgeniczebrafishrecapitulatingtbx16geneearlydevelopmentalexpression
AT michaellardelli transgeniczebrafishrecapitulatingtbx16geneearlydevelopmentalexpression
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